Sequences from confirmation amplicon


Confirmation sequences for line SALK_064044 and hit At5g53635 are shown.
Line ID SALK_064044
Locus At5g53635 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21787221
Primers used for PCR 40wu - LBb3, show primer details
   
Sequence Name 39-K118859-SALK_064044C-40wu
GenBank Accession LN485289 [GenBank]
Sequencing primer gene specific primer, 40wu: 5'-TATCTACGCCTCGAAATTGTTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K118859-SALK_064044C-40wu
TTTTTTTCTTTGCCGAAAGATTCTTGGAAAAAAACAACTGGTTGGTAGAGAGTTGAATCA
GGGATCGAAGGCAGATATGATAAGCCAATTACCTGATCCTTTGATCTGTCACATACTTTC
TCATCTTCCCATAAAGGACCTTGTTACGACACGCGTGTTATCCACCAGATGGAGGAGTCT
CTGGCTTTGGCTTCCTTGTTTGGAACTGAATTCCTTATACTTCCCAGATTTCAATGCCTT
TGTGAGTTTTGGTGACAAGTTTTTCGATTCCAATAGGGTTTCATGCATAAACAAGTTCAA
CATCATGCATTATCTCACATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTT
AATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCA
CCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAA
AATCCTGTTTGATGGTGGTTCCAA
   
Sequence Name 15-K118858-SALK_064044C-R210
GenBank Accession LN484710 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K118858-SALK_064044C-R210
TAATGCATGATGTTGAACTTGTTTATGCATGAAACCCTATTGGAATCGAAAAACTTGTCA
CCAAAACTCACAAAGGCATTGAAATCTGGGAAGTATAAGGAATTCAGTTCCAAACAAGGA
AGCCAAAGCCAGAGACTCCTCCATCTGGTGGATAACACGCGTGTCGTAACAAGGTCCTTT
ATGGGAAGATGAGAAAGTATGTGACAGATCAAAGGATCAGGTAATTGGCTTATCATATCT
GCCTTCGATCCCTGATTCAACTCTCTACCAACCAGTTGTTTTCTTTCCAAGAATCTATTC
GGCAAAAGAAAAAAAAACAAACTGAAAACGCAAGTCTTAAAGAAACAATTTCGAGGCGTA
GATA

Last Updated on 06.12.2018 16:11