Sequences from confirmation amplicon


Confirmation sequences for line SALK_064292 and hit At1g17050 are shown.
Line ID SALK_064292
Locus At1g17050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5831305
Primers used for PCR 35vz - LBb3, show primer details
   
Sequence Name 59-K118240-SALK_064292C-35vz
GenBank Accession LN485726 [GenBank]
Sequencing primer gene specific primer, 35vz: 5'-TGTTTTGTTTAGGTAATTCGGTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K118240-SALK_064292C-35vz
ACAATTTATAATTTATTCGGTTCCGAAAATTTTATGTTTCATTTTGAGTTAGTTAAAGAA
TCTCAATCAAAACCAAACTTAAATCCAACTAAATAGCAAGCTTATAAATATTACCATTTT
TTGTCCCAAAAATAAATAAATCAATATGACCATTTACAAAACTTCGATTTAATTTTTTTA
CTGTTTATGTGAAATTTGTTGTTTTCACTTTTCAATACCAAGATGTATAGAATTTTAACA
CAGATCAATTTATATTTGGGTCTAGTACCATTCACAAGTATCTAAAAAATTGAGTTACTT
TTATTTTGCAATAATAAGCATGAATTTTTGTTTATTACTCCATTATTGTGGTGTAAACAA
ATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTT
TTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGA
GTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGG
TTCCGAATGGGGCAAAA
   
Sequence Name 36-K118239-SALK_064292C-R210
GenBank Accession LN485243 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K118239-SALK_064292C-R210
TTTGTTTACCCACAATAATGGAGTAATAAACAAAAATTCATGCTTATTATTGCAAAATAA
AAGTAACTCAATTTTTTAGATACTTGTGAATGGTACTAGACCCAAATATAAATTGATCTG
TGTTAAAATTCTATACATCTTGGTATTGAAAAGTGAAAACAACAAATTTCACATAAACAG
TAAAAAAATTAAATCGAAGTTTTGTAAATGGTCATATTGATTTATTTATTTTTGGGACAA
AAAATGGTAATATTTATAAGCTTGCTATTTAGTTGGATTTAAGTTTGGTTTTGATTGAGA
TTCTTTAACTAACTCAAAATGAAACATAAAATTTTCTGGAACCGAATAAATTAATAAATT
TGTGGTTTCAATTCGAGAATGTTTGTCTGGACCGAATTACCTANACAAAAC

Last Updated on 06.12.2018 16:11