Sequences from confirmation amplicon


Confirmation sequences for line SALK_064531c and hit At3g26400 are shown.
Line ID SALK_064531c
Locus At3g26400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9667835
Primers used for PCR 85by - LBb3, show primer details
   
Sequence Name 17-K108287-SALK_064531C-85by
GenBank Accession LN484737 [GenBank]
Sequencing primer gene specific primer, 85by: 5'-AATCAGTCTAGGGTTTCGGATTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K108287-SALK_064531C-85by
TTGGGGGAAGAGAAGAACCACTTCCTTCTTTTGATCAAGGACGACAAGGTCGTTACAGTG
GCGATGGTGGTGGTTTTGGAGGTGGTGGAAGTG
   
Sequence Name 01-K108286-SALK_064531C-R210
GenBank Accession LN484268 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K108286-SALK_064531C-R210
GTCATTTGTTACCCACAGAAAGATAGAATTGGAAAAGATCCACAATCCTCAGTGACATTA
AAATAATCCAAATGAATAAGGACCTGTCAACCCTGCGATGCTCGAGTTCCAAATCCATCT
TACGCCAATCCTTCCCTTGTTCCTCTAACAACACTTCTCGAGGCTTTGCATCGCCAAAAG
GATTCACCTTTGGTCTCGGTTTCACCACATTATTCAATCCTGAACTCTCAGACCTGTTAG
ATTGAGCACTAGAAGGTCTACTAGAATGTGCACTCGTTGGCCTACTCGTTTGAGAACTTC
CTTTCTTAGCCTCAATCTCTGAGTCAATCTTCTTCCAATCCAAACCTTTCTCCGCCAAAA
CATCCTCCCTCGGTCTAGCTGCCCCAAATGGATTCGGCTTACTCGTCTTCTCAACAACAG
GTGGAGTCTCACTTCCTCCTGACTCAACCTTTCTTGGTTCCAAAACCAAACGACGACGTT
CTTCCTGACCTGAATCACCAAAACTCGACCCAAACGTAGATGATCTAACCGGTGCTTGCC
TTTTCCCTGCACCCCAATTATCAACATCATCAGCTCTAGATAACCCTCCACCTCCACCAC
CACCACCGCCTCCAAAACCACTTCCACCACCTCCAAAACCACCACCATCGCCACTGTAAC
GACCTTGTCGTCCTTGATCAAAAGAAGGAAGTGGTTTCTTCTCTTTCCCCCAATCATCAA
CCTCATCAGCTCTAGAAGGTTGAGGAAAATCCGAAACCCTAGACTGA

Last Updated on 06.12.2018 16:11