Sequences from confirmation amplicon


Confirmation sequences for line SALK_065267c and hit At1g72740 are shown.
Line ID SALK_065267c
Locus At1g72740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27380552
Primers used for PCR 06dx - LBb3, show primer details
   
Sequence Name 84-K110041-SALK_065267C-06dx
GenBank Accession LN486270 [GenBank]
Sequencing primer gene specific primer, 06dx: 5'-ATCCATCAGGAACAAAAATAGGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K110041-SALK_065267C-06dx
ACGAAGCTGCGCCAGCAACAATCAGACCTCTGCTGATTCACACAGATGATCGAGGAAGCT
GCAATAACCGCAGCCTGTAAAGTCGTAGAGGCGGAGAATAAAATAGACGTTGCCAAACTA
GCAGCAGAAGAGTTTGAGAAGATGACAAAGATTGCAGAAGAAAATAGAAAACTCCTGGTC
ATAGCTACTGAGATGCATGAACTATGTAAGAAAAAGCCAGACTCTTTTCTCATTATTGGC
TAAGCAAAACAAAACAAAACGTCTCTAACTTTGGTGTAAACAAATTGACGCTTAGACAAC
TTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAG
ACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCC
ACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCGGGCAAAA
   
Sequence Name 77-K110040-SALK_065267C-R210
GenBank Accession LN486169 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K110040-SALK_065267C-R210
GTCACTTTGTTTACACCAAAGTTAGAGACGTTTTGTTTTGTTTTGCTTAGCCAATAATGA
GAAAAGAGTCTGGCTTTTTCTTACATAGTTCATGCATCTCAGTAGCTATGACCAGGAGTT
TTCTATTTTCTTCTGCAATCTTTGTCATCTTCTCAAACTCTTCTGCTGCTAGTTTGGCAA
CGTCTATTTTATTCTCCGCCTCTACGACTTTACAGGCTGCGGTTATTGCAGCTTCCTCGA
TCATCTGTTGTGAATCAGCAGAGGTCTGATTGTTTGCTTGGCGCAGCTTCGTATGTGTCT
CCTTTGGTTTTGGGACACCTATTTTTGTTCCTGATGGA

Last Updated on 06.12.2018 16:11