Sequences from confirmation amplicon


Confirmation sequences for line SALK_065342 and hit At5g27430 are shown.
Line ID SALK_065342
Locus At5g27430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9688493
Primers used for PCR 15ii - LBb3, show primer details
   
Sequence Name 26-K112623-SALK_065342C-15ii
GenBank Accession LN485033 [GenBank]
Sequencing primer gene specific primer, 15ii: 5'-GGATTGTTCTGGAAGTTTGTTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K112623-SALK_065342C-15ii
ATTTTTTTATGATTGTTCTGTGGTTTGCAGATATTGAATATCAATTGGTTTCAGAAGCAA
CCTCATGGAAATGATGAGGTAAGGTTTCATACTCTTGCCTCTTCTTACGGCTTTCTGTGT
CTTCACTGTAAGTTTCTATGATTTGAGCCACCAATATATATGCTCTGGTGTGCTGAGTTA
TGTTTATCTGGTCACGCTTAGTGGGTAAAATTATGCTTATTTTAGCATAAACTTTAATGA
GATTAGGTTTTGTATCACACCGATCTTTAGTTGTTTAGTAAGATGACAGAAATTCTTGGT
AAAACACTCTAAATCGTCTTCTTTAGTGAAGTTTTCCTTAGAGTAGCATAAATTTTGGCT
TTTTTCTTGATGGTTGAATAAGGTGGCACTTGTTGGTATGAGACTTTATTGAGAGTCATA
TTAAGCTTAGCGTTCATTGAATTTGATGGCCATAGGGGTTTAGATGCAACTGTTTCTTTG
AACATTGTAGAAATATATAAAGATTTTACATTAGCCTACTCTTGAAAGTCAAATTGTCGA
ATTTGATTATATTATACTCTAGAGGTGATATTAGTTAATGAGTTTATACTCGGTTATTTA
CAGCTTATTCATATACCAGTTAACGTGTCTCATATATTCTAACTTCTTAGCATTTAACGT
GTTTGCAGGTCAGCTTGACACTGAACATAACAGCTGACTTGCCCCAAATAAACAAATTGA
CGCTNAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTT
TTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGC
AGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 02-K112622-SALK_065342C-R210
GenBank Accession LN484307 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K112622-SALK_065342C-R210
CGTCATTTGTTATTTGGGGCAGTCAGCTGTTATGTTCAGTGTCAAGCTGACCTGCAAACA
CGTTAAATGCTAAGAAGTTAGAATATATGAGACACGTTAACTGGTATATGAATAAGCTGT
AAATAACCGAGTATAAACTCATTAACTAATATCACCTCTAGAGTATAATATAATCAAATT
CGACAATTTGACTTTCAAGAGTAGGCTAATGTAAAATCTTTATATATTTCTACAATGTTC
AAAGAAACAGTTGCATCTAAACCCCTATGGCCATCAAATTCAATGAACGCTAAGCTTAAT
ATGACTCTCAATAAAGTCTCATACCAACAAGTGCCACCTTATTCAACCATCAAGAAAAAA
GCCAAAATTTATGCTACTCTAAGGAAAACTTCACTAAAGAAGACGATTTAGAGTGTTTTA
CCAAGAATTTCTGTCATCTTACTAAACAACTAAAGATCGGTGTGATACAAAACCTAATCT
CATTAAAGTTTATGCTAAAATAAGCATAATTTTACCCACTAAGCGTGACCAGATAAACAT
AACTCAGCACACCAGAGCATATATATTGGTGGCTCAAATCATAGAAACTTACAGTGAAGA
CACAGAAAGCCGTAAGAAGAGGCAAGAGTATGAAACCTTACCTCATCATTTCCATGAGGT
TGCTTCTGAAACCAATTGATATTCAATATCTGCAAACCACAGAACAATCATTAAAAAAAT
CAGGCTCCACAAAAGTTTCTTCAAACTCAAAGAACAAACTTC

Last Updated on 06.12.2018 16:11