Sequences from confirmation amplicon


Confirmation sequences for line SALK_065859c and hit At4g39960 are shown.
Line ID SALK_065859c
Locus At4g39960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18535070
Primers used for PCR 69kh - LBb3, show primer details
   
Sequence Name 72-K113914-SALK_065859C-69kh
GenBank Accession LN486059 [GenBank]
Sequencing primer gene specific primer, 69kh: 5'-GTGTGTTCAATTTCCAGGATTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K113914-SALK_065859C-69kh
GCGGTATGCAATTTCTATTTTACTGAGTTCCCTAATCAGGAAAAACGCAACACTCATGTG
TAGCATCGACGTTTCTCTTTGATGAGCTTCACGCGTTCAGCTTTTAAGTTGTGCTTTTCT
ATAGTTTGACAATGATTCTACTCTGCACCTATTTTGCAGCTTATCGGAAGCTCGCTAGGA
GTTATCATCCAGATGTGAACAAGTAAGTGAAGGGTTTAGTTTACTTTGTATCCAGTATAA
GGTCTTCGTCTGTTTATATTATTTACCTTCCTGCATCAAGATTGGTGTGTTTCTTGTTAC
AACTCGATTACAATATTTGAGAAACGCTCAGTCTAGAAAATTATTGCAATTCGTTGGTTA
AGTCTCTCCTTATATGAACGAACATACGACCACCAACCTGTAAAATTTTGATGAAGAATT
TCTGAAACTGCTTCAAAAATTGTGGTGTTTACAAATTGACGCTTAGACAACTTAATAACA
CATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAAC
AGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTT
TGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTT
   
Sequence Name 61-K113913-SALK_065859C-R210
GenBank Accession LN485768 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K113913-SALK_065859C-R210
CGTTCATATAAGGAGAGACTTAACCAACGAATCGCAATAATTTTCTAGACTGAGCGTTTC
TCAAATATTGTAATCGAGTTGTAACAAGAAACACACCAATCTTGATGCAGGAAGGTAAAT
AATATAAACAGACGAAGACCTTATACTGGATACAAAGTAAACTAAACCCTTCACTTACTT
GTTCACATCTGGATGATAACTCCTAGCGAGCTTCCGATAAGCTGCAAAATAGGTGCAGAG
TAGAATCATTGTCAAACTATAGAAAAGCACAACTTAAAAGCTGAACACATGAAGCTCATC
AAAGAGAAACATCAATGCTACACATGAGTGTTGCATTTTCCTGATTAGGGAACTCAGTGA
AATAGAATTGCATACCGCTTTTAATCTCAGCTTTGGTTGCATTTTTCGAGACTCCAAGGA
CAGAATAGAAATCCT

Last Updated on 06.12.2018 16:11