Sequences from confirmation amplicon


Confirmation sequences for line SALK_066347c and hit At5g64560 are shown.
Line ID SALK_066347c
Locus At5g64560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25807194
Primers used for PCR 44kq - LBb3, show primer details
   
Sequence Name 73-K113914-SALK_066347C-44kq
GenBank Accession LN486080 [GenBank]
Sequencing primer gene specific primer, 44kq: 5'-CACGTCTTGATATCTTTACAGGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K113914-SALK_066347C-44kq
CCCTTTTCCTTTCCGTTCTTTTTTGCAGAACTTTTTTTACTTTAAAAACTTAATTTTTGA
CTCTGATGCAGCTACGTGAGTATATTGATGACACAGAGGACTACATTAACATCCAGGTAT
CCAAAAACGAAACCTCAAAATTTTTAACATGTTCTTGAAATCTGAATATAAGTCATTATA
TTAAAAGCTAAAACTCGTCGGTTTTCTATTCACAGCTTGACAATCATCGGAATCAGCTTA
TTCAGGTACGTGAATGTCGAAAGCCAAAGTTTTTTTAATATGTCATCTTCTTTTGTTGAT
CATCTTCAAAATCCTGCAGTTGGAGCTAGTGTTAAGTTCTGGAACAGTTTGCTTATCGAT
GTACTCTCTTGTCGCTGGAATTTTCGGGATGAACATTCCATACACATGGAACGACGGTCA
TGGATACATGTTCAAATACGTAAGAAACCAGAATTCTATTCGCATGAGGATGAAATAAAG
ATCTCTCTCAAGATTAACTTATGTTTAGACAAATTGACGCTTAGACNACTTAATAACACA
TTGCGGACGTTTTTAATGTACTGGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACA
GCTGATTGCCCTTCACCGCCNGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTT
GCCCCAGCAGGCGAAAATCCTGTTTGATGGTGG
   
Sequence Name 62-K113913-SALK_066347C-R210
GenBank Accession LN485796 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K113913-SALK_066347C-R210
TCGGAGAGAGAATTTTCTTTTATTCCTTCAGGCGAAATAGAAATTTCCGGTTTTCTTACG
TATTTGAAACACGTATCCATGACCGTCGTTTCATGTGTATCGGAATCGTTCATCCCGAAA
ATTCCAGCGACAAGAGAGTACATCGATAAGCAAACTGTTCCAGAACTTAACACTAGCTCC
AACTGCAGGATTTTGAAGATGATCAACAAAAGAAGATGACATATTAAAAAAACTTTGGCT
TTCGACATTCACGTACCTGAATAAGCTGATTCCGATGATTGTCAAGCTGTGAATAGAAAA
CCGACGAGTTTTAGCTTTTAATATAATGACTTATATTCAGATTTCAAGAACATGTTAAAA
ATTTTGAGGTTTCGTTTTTGGATACCTGGATGTTAATGTAGTCCTCTGTGTCATCAATAT
ACTCACGTAGCTGCATCAGAGTCAAAAATTAAGTTTTTAAAGTAAAAAAAGTCTGCAAAA
AAGAACAGAAAGAAAAGGGAGCAAGAGGATAACTGTTGTTAATCTGTTCAAAGTGCTGTC
GATCTGCATGAAGTATGCCTGTAA

Last Updated on 06.12.2018 16:11