Sequences from confirmation amplicon


Confirmation sequences for line SALK_066908c and hit At5g15410 are shown.
Line ID SALK_066908c
Locus At5g15410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5005735
Primers used for PCR 17ey - LBb3, show primer details
   
Sequence Name 79-K111509-SALK_066908C-17ey
GenBank Accession LN486206 [GenBank]
Sequencing primer gene specific primer, 17ey: 5'-CGATGAAAACAGTAACCTCCAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K111509-SALK_066908C-17ey
ACGGCGCGATGAGACGCCGGTGCTAAGCTCCGTCGAGTGTTACGCTTGCACACAAGTAGG
CGTCCCAGCTTTCCATTCAACTAGCTGCGATCAAGCTCACGCGCCGGAGTGGCGTGCCTC
CGCCGGCTCTTCTCTAGTTCCGATCCAGGAAGGATCTGTCCCTAACCCAGCCCGAACCAG
ATTCCGACGTCTCAAAGGTCCGTTTGGTGAAGTTCTCGATCCTAGGAGCAAGCGCGTGCA
GAGATGGAACCGCGCGTTGCTTTTAGCTCGTGGGATGGCTTTAGCGGTGGATCCGCTCTT
CTTCTACGCGCTTTCCATCGGCCGAACTACCGGACCGGCGTGTCTTTACATGGATGGTGC
GTTCGCCGCGGTGGTCACGGTGCTCCGCACGTGTCTCGATGCTGTTCATCTTTGGCACGT
GTGGCTTCAATTCAGACTGGCCTACGTCTCGAGAGAGTCGCTTGTCGTTGGTTGTGGGAA
GCTCGTTTGGGATCCACGCGCCATCGCGTCTCACTACGCACGCTCTCTCAAACAAATTGA
CGCTNAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTT
TTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGC
AGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTC
   
Sequence Name 65-K111508-SALK_066908C-R210
GenBank Accession LN485871 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K111508-SALK_066908C-R210
GTCATTTGTTTGAAGAGCGTGCGTAGTGAGACGCGATGGCGCGTGGATCCCAAACGAGCT
TCCCACAACCAACGACAAGCGACTCTCTCGAGACGTAGGCCAGTCTGAATTGAAGCCACA
CGTGCCAAAGATGAACAGCATCGAGACACGTGCGGAGCACCGTGACCACCGCGGCGAACG
CACCATCCATGTAAAGACACGCCGGTCCGGTAGTTCGGCCGATGGAAAGCGCGTAGAAGA
AGAGCGGATCCACCGCTAAAGCCATCCCACGAGCTAAAAGCAACGCGCGGTTCCATCTCT
GCACGCGCTTGCTCCTAGGATCGAGAACTTCACCAAACGGACCTTTGAGACGTCGGAATC
TGGTTCGGGCTGGGTTAGGGACAGATCCTTCCTGGATCGGAACTAGAGAAGAGCCGGCGG
AGGCACGCCACTCCGGCGCGTGAGCTTGATCGCAGCTAGTTGAATGGAAAGCTGGGACGC
CTACTTGTGTGCAAGCGTAACACTCGACGGAGCTTAGCACCGGCGTCTCATCGCTGCCGC
TGCTGCTCGAATCTCCACCGTTGATTTGGAGGTTACTGTTTTCATC

Last Updated on 06.12.2018 16:11