Sequences from confirmation amplicon


Confirmation sequences for line SALK_066923c and hit At2g20030 are shown.
Line ID SALK_066923c
Locus At2g20030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8648945
Primers used for PCR 34ux - LBb3, show primer details
   
Sequence Name 26-K117668-SALK_066923C-R210
GenBank Accession LN485045 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K117668-SALK_066923C-R210
TTTGTTTACCACAATATATCCTTTTCTCTCTGTTAACAAACCACTGAGCCGTTCTTCTAG
CGATCGGCAGCCATAACCGCCGTCTTCTTTCTTCCGTGGTCGCAGTAACGGCATAATTCT
GCGAAGTGGACGCGGTTGAACCTCTGTAGCTTCCTCCAACCGCCTTTTCTCTTGAAACAG
TCGTGATCTCAGAAACCGTTCTTCTGGATGAGTCTTTGTGCTTAATCAACATTCTTTTCA
TTTCCATATCTTCCTTGTCCCGCAAGTTACCTCTATGCACTCGATCCACCGACGAAAATC
TGTCGCTTGACACCGAATTCAGCATTTCTGATGTCAAAAACGTCAAATCAGATGAA
   
Sequence Name 63-K117669-SALK_066923C-34ux
GenBank Accession LN485829 [GenBank]
Sequencing primer gene specific primer, 34ux: 5'-CTTCATCTGATTTGACGTTTTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K117669-SALK_066923C-34ux
GCGACGATTTTCGTCGGTGGATCGAGTGCATAGAGGTAACTTGCGGGACACCGATGATAT
GGAAATGAAAAGAATGTTGATTAAGCACAAAGACTCATCCAGAAGAACGGTTTCTGAGAT
CACGACTGTTTCAAGAGAAAAGGCGGTTGGAGGAAGCTACAGAGGTTCAACCGCGTCCAC
TTCGCAGAATTATGCCGTTACTGCGACCACGGAAGAAAGAAGACGGCGGTTATGGCTGCC
GATCGCTAGAAGAACGGCTCAGTGGTTTGTTAACAGAGAGAAAAGGATATATTGTGGTGT
AAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGG
TGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCT
GAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGA
TGGTGGTTCCGAAATCGGCAAAA

Last Updated on 06.12.2018 16:11