Sequences from confirmation amplicon


Confirmation sequences for line SALK_067271c and hit At1g61790 are shown.
Line ID SALK_067271c
Locus At1g61790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22814427
Primers used for PCR 36lm - LBb3, show primer details
   
Sequence Name 76-K114866-SALK_067271C-36lm
GenBank Accession LN486157 [GenBank]
Sequencing primer gene specific primer, 36lm: 5'-CCTGATAATGTTGTGCATTGTACC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K114866-SALK_067271C-36lm
GAATAGTTCACTCCGTTCAAAACTCTGGTTGATGATAACCGGAGTAGCGATAACGATCAT
CGCAGCGATAATACCGATCTGTGGTTTCCAAAGTCTCGGCGGACGTTGAATAGGACCGAC
CCTGACTTTAGTTCGTTGCTCATTAAACTCACCCATTGATTCAGCTAATCTACAGTAATC
CGATTGATCCATTTGACCAGATTCGTCACGTAGATTCGATATCGAAAGACTTACAAGACG
AATGTGAGGCAAAGCGTTAACGCCAAAGAGCTGGAACGAAGATTGAGACTTCGAAAACTC
TATCTCACTGAACAAAAGCTTAGTTCCTTCATATCCATTGTTGTTAGCGAGGAATGAAGC
GGATACGATGCCGAATTCGCGACGGAGCTCTTGAAGACGAAGCTCGTTTTTGCTGTGGAG
TTGAGTAGCGTCTAAGAAGACGAGTAACGAGTTAGGACGAGGCGTGGAAGCGGAGGTTAG
GAATTTTGAGATTCCATGGTCATCGAGATGGATTACGCCTGATTCGCTTGTTGATCTGAG
AGATACAAGTTCGTTGAGAAGATCTGAATCGGAATCCGAATCGGAAAACGAAGCTGCGAA
TGTGGATGATAGTGTTGCTATGACAAATTGATATATTGTGGTGTAAACAAATTGACGCTT
AGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTT
   
Sequence Name 36-K114865-SALK_067271C-R210
GenBank Accession LN485236 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K114865-SALK_067271C-R210
CGTCATTTGTTTCACCACAATATATCAATTTGTCATAGCAACACTATCATCCACATTCGC
AGCTTCGTTTTCCGATTCGGATTCCGATTCAGATCTTCTCAACGAACTTGTATCTCTCAG
ATCAACAAGCGAATCAGGCGTAATCCATCTCGATGACCATGGAATCTCAAAATTCCTAAC
CTCCGCTTCCACGCCTCGTCCTTACTCGTTACTCGTCTTCTTCGACGCTACTCAACTCCA
CAGCAAAAACGAGCTTCGTCTTCAAGAGCTCCGTCGCGAATTCGGCATCGTCTCCGCTTC
ATTCCTCGCTAACAACAATGGATCTGAAGGAACTAAGCTTTTCTTCTGTGAGATCGAGTT
TTCGAAGTCTCAATCTTCGTTCCAGCTCTTTGGCGTTAACGCTTTACCTCACATTCGTCT
TGTAAGTCCTTCGATATCGAATCTACGTGATGAATCTGGTCAAATGGATCAATCGGATTA
CTCTAGATTAGCTGAATCAATGGCTGAGTTTGTTGAGCAACGAACTAAACTCAAGGTCGG
TCCTATTCAACGTCCACCGCTACTTTCGAAACCACAGATCGGTATTATCGTTGCGTTGAT
CGTTATCGCTACTCCGTTTATCATCAAAAGAGTTTTGAAAGGAGAAACTATTCTTCATGA
TACTAGACTTTGGTTATCTGGTGCTATCTTCATTTACTTCTTTAGTGTTGCTGGTACAAT
GCACAACATTATCA

Last Updated on 06.12.2018 16:11