Sequences from confirmation amplicon


Confirmation sequences for line SALK_067473c and hit At1g06040 are shown.
Line ID SALK_067473c
Locus At1g06040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1829357
Primers used for PCR 86by - LBb3, show primer details
   
Sequence Name 18-K108287-SALK_067473C-86by
GenBank Accession LN484770 [GenBank]
Sequencing primer gene specific primer, 86by: 5'-CATCAAAGCATTAAGCATAGACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K108287-SALK_067473C-86by
ACTCAACACAGACATTTGTTATAAGCTCTCAACATATCTTACACAACTTAAGACAAAACT
AAAATGTATATGACATTAACTCCACACATGCAGATAGGTTTCTTGTTTGTTCTTTTACAT
TGAAGAATGTCTACACATAGATTACATATAGCTTTTTAGCCAAGATCAGGGACAATGAAG
TGTTCCTCATCATCATCATCATATCTGGTCTCAAACCTCGGCTTCTTGTGTGAAACATTT
GACTTCATAGGTTTGTATGAATGAACATGACCTAAATGCGAAACAGAAAGCTCAGGAACT
TCAGCTGCAGGAAGAGCCTTGTCATTAATCTGATCACCGAAGAATCCCATGTCTGAAAAC
CAATCCAACTCCCCTGCCCCAAGATCAAGCTGTCCTTTCTGCTCAATTTAAACCAAGAAG
TCAATAAAAGCTTCTTGCAAAGAAAGTGATCTAACATTGAATCTCAATTCTCATCACTAC
CTTGTCGGTGGATTCAATATCAGAGAAGTGAAAGAAATCGTCAACAGCCCAGGGAAGTGG
AGTAGCAGAAGAAGGTTGTTGTTGCTGCTGGCTTGTGGATTTAGCAGGAATCTGATTAGC
CTTCTGTTGGTTGTTGGAAGGCTCAGGTTGATTCTTCTCAATTTCTTTACTACATATAGT
TGAGGTCAGAGCTACTTTGATCCCAGTGGCTAAGAACCTCTGGTGATTAGCAGATCGAGA
ATTAGCCACGTGGATGGATTCATCGCAGTCCCTGCAAAGCAGAGCTCTATCCTCTACACA
GAAAATGAAAGCTGCCTTCTCCTACAAGTCATAAGAACATAATGAGCAAACCCTAGTCTT
AAACAACAAGGTATTAACCTTGCAAACAGATCTATAAAATCACAACTTTAATGCCTTAAT
AACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGG
CAACAGCTGATTGCCT
   
Sequence Name 02-K108286-SALK_067473C-R210
GenBank Accession LN484298 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K108286-SALK_067473C-R210
TAGTGTGATTTATGATCTTGTTGCAAGGTTAATACCTTGTTGTTTAGACTAGGGTTTCGC
TTTTATGTTCTTATGACTTGTAGGAGAAGGCAGCTTTCATTTTCTGTGTAGAGGATAGAG
CTCTGCTTTGCAGGGACTGCGATGAATCCATCCACGTGGCTAATTCTCGATCTGCTAATC
ACCAGAGGTTCTTAGCCACTGGGATCAAAGTAGCTCTGACCTCAACTATATGTAGTAAAG
AAATTGAGAAGAATCAACCTGAGCCTTCCAACAACCAACAGAAGGCTAATCAGATTCCTG
CTAAATCCACAAGCCAGCAGCAACAACAACCTTCTTCTGCTACTCCACTTCCCTGGGCTG
TTGACGATTTCTTTCACTTCTCTGATATTGAATCCACCGACAAGGTAGTGATGAGAATTG
AGATTCAATGTTAGATCACTTTCTTTGCAAGAAGCTTTTATTGACTTCTTGGTTTAAATT
GAGCAGAAAGGACAGCTTGATCTTGGGGCAGGGGAGTTGGATTGGTTTTCAGACATGGGA
TTCTTCGGTGATCAGATTAATGACAAGGCTCTTCCTGCAGCTGAAGTTCCTGAGCTTTCT
GTTTCGCATTTAGGTCATGTTCATTCATACAAACCTATGAAGTCAAATGTTTCACACAAG
AAGCCGAGGTTTGAGACCAGATATGATGATGATGATGAGGAACACTTCATTGTCCCTGAT
CTTGGCTAAAAAGCTATATGTAATCTATGTGTAGACATTCTTCAATGTAAAAGAACAAAC
AAGAAACCTATCTGCATGTGTGGAGTTAATGTCATATACATTTTAGTTTTGTCTTAAGTT
GTGTAAGATATGTTGAGAGCTTATAACAAATGTCTGTGTTTGAGTTTTGTTCATACCTCT
TGAACATGGTCTATGCTT

Last Updated on 06.12.2018 16:11