Sequences from confirmation amplicon


Confirmation sequences for line SALK_067618c and hit At2g32050 are shown.
Line ID SALK_067618c
Locus At2g32050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13638006
Primers used for PCR 88ns - LBb3, show primer details
   
Sequence Name 67-K114703-SALK_067618C-88ns
GenBank Accession LN485936 [GenBank]
Sequencing primer gene specific primer, 88ns: 5'-ACCGAGCTGATATTGAAGACTGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K114703-SALK_067618C-88ns
CACTTGTGTTATGATCAACACGAGGAGGAGGAGCAAGCCGAGGACGGTGGGGATAGGATG
AGTTCTTTAGAGAAGAGAACTTTGGTTTCGAAAAGAGAGGTTGATGTAATGGCTGCACTT
GATGAGATGAAATCTATGAAATCTAGACGCGTATCGGTGAGTGTAGACTCTATGCTTGAG
GATTTGAGTAAAAGACACAAAGAAGAAGAAGAAGTTGCAAAGGAGGAAGATGCAGCGTTG
ATAAAGTCTACCAAATTTGGTAAGCAGAGGAGGATTGTCGACGAAGAAACTGATGAGATG
GAGAAGACGAAGAAAGTAAGAATGGACACAGGATATATTGTGGTGTAAACAAATTGACGC
TTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTC
ACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC
AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGA
   
Sequence Name 58-K114702-SALK_067618C-R210
GenBank Accession LN485695 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K114702-SALK_067618C-R210
ACCACAATATATCCTGTGTCCATTCTTACTTTCTTCGTCTTCTCCATCTCATCAGTTTCT
TCGTCGACAATCCTCCTCTGCTTACCAAATTTGGTAGACTTTATCAACGCTGCATCTTCC
TCCTTTGCAACTTCTTCTTCTTCTTTGTGTCTTTTACTCAAATCCTCAAGCATAGAGTCT
ACACTCACCGATACGCGTCTAGATTTCATAGATTTCATCTCATCAAGTGCAGCCATTACA
TCAACCTCTCTTTTCGAAACCAAAGTTCTCTTCTCTAAAGAACTCATCCTATCCCCACCG
TCCTCGGCTTGCTCCTCCTCCTCGTGTTGATCATAAACACAAGTTGCACCTGATTCGGCT
ACATAACTCGAATTCTTTGGATCAGTCTTCAATACAAACTCGG

Last Updated on 06.12.2018 16:11