Sequences from confirmation amplicon


Confirmation sequences for line SALK_067994c and hit At1g16460 are shown.
Line ID SALK_067994c
Locus At1g16460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5620083
Primers used for PCR 35gz - LBb3, show primer details
   
Sequence Name 78-K112669-SALK_067994C-35gz
GenBank Accession LN486188 [GenBank]
Sequencing primer gene specific primer, 35gz: 5'-ATCAATCTCCACAAAGATGAGACC-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K112669-SALK_067994C-35gz
CCCTCCCGCAATTTGTTAAGTTTAACACAGAAATGGATTGAACCAAGCTAAGCTTATAAA
TATATATCCACTGGCTTGTGATCTTGTAGCTTTTTCTTGGACTTTCTTATGAAGAAGAAC
CCACTATGGGCAAGTTTGGTGCAGTTGCCCATTCAGTCCAAGACCCATCATAGATTGCCA
CATTAGTTTTTCCCAGACGATAAAGTCCCTGACCAGTAAAATAAAGAGGAATCAGTCTTT
AGTTTTTATCATATGATCACAGTAAATGAATGAATTCTAGATCATCATTTAGTTACCAAT
GCCAAAATACAAGCTGTTACACCGGTTCCACATGAGGCCGCAATCGGACTGTGGGACAAC
TTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAG
ACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCC
ACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGA
   
Sequence Name 68-K112668-SALK_067994C-R210
GenBank Accession LN485955 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K112668-SALK_067994C-R210
ATGCGGCCTATGTGGAACGGTGTAACAGCTTGTATTTTGGCATTGGTAACTAAATGATGA
TCTAGAATTCATTCATTTACTGTGATCATATGATAAAAACTAAAGACTGATTCCTCTTTA
TTTTACTGGTCAGGGACTTTATCGTCTGGGAAAAACTAATGTGGCAATCTATGATGGGTC
TTGGACTGAATGGGCAACTGCACCAAACTTGCCCATAGTGGGTTCTTCTTCATAAGAAAG
TCCAAGAAAAAGCTACAAGATCACAAGCCAGTGGATATATATTTATAAGCTTAGCTTGGT
TCAATCCATTTCTGTGTTAAACTTTAACAAATTGCTGGGAGGGTTGTATTAGTATTATAC
ATCAGTATTCAGGATTCTTGAGTTTGGTCTCATCTTTGTGGAGATTGA

Last Updated on 06.12.2018 16:11