Sequences from confirmation amplicon


Confirmation sequences for line SALK_068236 and hit At1g53770 are shown.
Line ID SALK_068236
Locus At1g53770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20072119
Primers used for PCR 23hd - LBb3, show primer details
   
Sequence Name 69-K112564-SALK_068236C-23hd
GenBank Accession LN485977 [GenBank]
Sequencing primer gene specific primer, 23hd: 5'-GGGGATGTGTTCTATGCTAATGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K112564-SALK_068236C-23hd
GCTCACAGTGCAGAACTTTGATTGAACAAACCGGCTTATTTTACTCACTGCACAGAGATT
TATCCAGACTTTCCTTGGCAAGAACTACATTGCTCTTCATTTCCGCAGGCATGGATTCTT
GAAGTTTTGGTATGATCTATTTTCTACTTCTCTGGTAGTAGCCGTAGATGGTAACTGAAA
GTGAGAATTTTATGCTTGACCTTCACTCTTCTAAGTTCTCTTGTATAATGGTAAAGAACT
TTGCGCATAGCTACACTCTACATTTGATGACAATGTAGGTATCTTAATCTTGATCAGATA
ATAGCAGTATTGGTATAGTAACCATTTAGAATTGCGGCTGTACTTTTGTGAATAGATAAT
TGGAGTTGCACATCTAACTAGCTTAGGCGAATTCCATTGACCAAGTTATCATTTATGAAT
AAGTATGAGCATTATGAGTTTATATCACTTGTACCAGGATATATTGTGGTGTAAACAAAT
TGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTT
CTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGT
TGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTT
CCGA
   
Sequence Name 53-K112563-SALK_068236C-R210
GenBank Accession LN485583 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K112563-SALK_068236C-R210
TCATTTGTTACACCACAATATATCCTGGGTACAGTGATATAAACTCATAATGCTCATACT
TATTCATAAATGATAACTTGGTCAATGGAATTCGCCTAAGCTAGTTAGATGTGCAACTCC
AATTATCTATTCACAAAAGTACAGCCGCAATTCTAAATGGTTACTATACCAATACTGCTA
TTATCTGATCAAGATTAAGATACCTACATTGTCATCAAATGTAGAGTGTAGCTATGCGCA
AAGTTCTTTACCATTATACAAGAGAACTTAGAAGAGTGAAGGTCAAGCATAAAATTCTCA
CTTTCAGTTACCATCTACGGCTACTACCAGAGAAGTAGAAAATAGATCATACCAAAACTT
CAAGAATCCATGCCTGCGGAAATGAAGAGCAATGTAGTTCTTGCCAAGGAAAGTCTGGAT
AAATCTCTGTGCAGTGAGTAAAATAAGCCGGTTTGGTTCAATCAAAGTTCTGCACTTGTG
AGCAACAGGACCACCAGGCTGCATCACCCACTCTCTCTCAACATTAGCATAGAACACATC
CC

Last Updated on 06.12.2018 16:11