Sequences from confirmation amplicon


Confirmation sequences for line SALK_069046c and hit At3g04030 are shown.
Line ID SALK_069046c
Locus At3g04030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1043636
Primers used for PCR 41um - LBb3, show primer details
   
Sequence Name 84-K117830-SALK_069046C-41um
GenBank Accession LN486276 [GenBank]
Sequencing primer gene specific primer, 41um: 5'-CGAAATTCAAAGCGAGAATTTAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K117830-SALK_069046C-41um
ATATGATATTTGTCTATATACCATCTAATGTATTAGCTTATCACAAACTGACCAAATCTG
TTTACACACATAGGAATTCACCAATAGGTGAAGCACTGCAAATGCAAATAGAGGTCCAAA
GAAGGCTTCATGAGCAACTTGAGGTATCATATTTCTACAACACTGTTCTTTACTCCAATT
AAACACGTTAAAGATTCTTGCAGTTCATAAAGCACTTAGAATTATCAGTCAGTGTTTAGA
GGATAAGTTTGTAGTATGTAGAAATATAAAAGCATTCAGGATATATTGTGGTGTAAACAA
ATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTT
TTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGA
GTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGG
TTCCAAATCGGGCAAAA
   
Sequence Name 67-K117829-SALK_069046C-R210
GenBank Accession LN485939 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K117829-SALK_069046C-R210
CATTTGTTTACCCACAATATATCCTGAATGCTTTTATATTTCTACATACTACAAACTTAT
CCTCTAAACACTGACTGATAATTCTAAGTGCTTTATGAACTGCAAGAATCTTTAACGTGT
TTAATTGGAGTAAAGAACAGTGTTGTAGAAATATGATACCTCAAGTTGCTCATGAAGCCT
TCTTTGGACCTCTATTTGCATTTGCAGTGCTTCACCTATTGGTGAATTCCTATGTGTGTA
AACAGATTTGGTCAGTTTGTGATAAGCTAATACATTAGATGGTATATAGACAAATATTCA
TATTTATACTTGTTTGGCTGTGGTCCAATGCTTAAATTCTCGCTTTGAATTTCG

Last Updated on 06.12.2018 16:11