Sequences from confirmation amplicon


Confirmation sequences for line SALK_069091c and hit At5g57360 are shown.
Line ID SALK_069091c
Locus At5g57360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23243415
Primers used for PCR 22ht - LBb3, show primer details
   
Sequence Name 27-K112874-SALK_069091C-22ht
GenBank Accession LN485063 [GenBank]
Sequencing primer gene specific primer, 22ht: 5'-ATCTTTCTTCCTCCATAAACCGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K112874-SALK_069091C-22ht
CAGCTGGATCTCTCTCCACACTGGTTTCTCTATTGAAAGGTCGAGGAGGAACGTGTCACT
TAGAAGAACGCCGGAATCTGCACAGCCTCCAGAGACTATCAGTTTGGTTCCGTCAAGGGT
GCATGAGCTGTGCCATGATCTTGGTAGAGGCGGAGCCAAACCCGAAATTTCCCTCCAAGT
AGGCGGCTTAGCATCGAGATTCAAGACAAACACATCGTTCAGCAAACCTTGCTGACCACA
GCCACCAAATACAACCAGATTGGAGCCATTTACACAAGTGAGCGTGTGGCCCCAGCGTCC
TGGAGGAGGGGAGCTCACTTTGACATGTTGCCATTCAGGGTAATCAGAATTCAGATCTAA
AACAAAGGTATCATTCATAGGCTGCATATTCACACCTTCTCCTCCAAACAGAACAACTCT
GTTCCCGACAGCACAAGCACTAAAATTACACATTGCGGACGTTTTTAATGTACTGGGGTG
GTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGA
GAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATG
GTGGTTCCGAATCGGGCAAAA
   
Sequence Name 19-K112873-SALK_069091C-R210
GenBank Accession LN484817 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K112873-SALK_069091C-R210
ACCCTGAATGGGAACCTGTCAAAGTGAGCTCCCCTCCTCCAGGACGCTGGGGCCACACGC
TCACTTGTATAAATGGCTCCAATCTGGTTGTATTTGGTGGCTGTGGTCAGCAAGGTTTGC
TGAACGATGTGTTTGTCTTGAATCTCGATGCTAAGCCGCCTACTTGGAGGGAAATTTCGG
GTTTGGCTCCGCCTCTACCAAGATCATGGCACAGCTCATGCACCCTTGACGGAACCAAAC
TGATAGTCTCTGGAGGCTGTGCACATTCCGGCGATCTTCTAAATGACACGTTCCTCCTCG
ACCTTTCTATAGAGAAACCAGTGTGGAGAGAGATTCCAGCTGCCTGGACTCCGCCTTCCC
GGTTAGGCCACACACTATCTGTTTATGGAGGAAGAAAGAT

Last Updated on 06.12.2018 16:11