Sequences from confirmation amplicon


Confirmation sequences for line SALK_069427c and hit At3g04090 are shown.
Line ID SALK_069427c
Locus At3g04090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1072800
Primers used for PCR 23md - LBb3, show primer details
   
Sequence Name 32-K115696-SALK_069427C-23md
GenBank Accession LN485171 [GenBank]
Sequencing primer gene specific primer, 23md: 5'-TAGCCTTTTCTAGAGTTTCTTGCG-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K115696-SALK_069427C-23md
ATAGAAGACTGTTAAATTTGGTAACCTGATTTAGCAAGGAGTAATTTGTACTCTTTTTGA
GAGTTGAATCAAGCCAGATGACTTTATTGCCTTTGGTAGGAATAGTCTTCATGATCCCTT
TTTTGCCACTGTTTCTTGATTTCGACGGGATAATGTTTGAAAGGAGATGTGATATATTAT
ATCTGTTATTCCAGGCAATTGGTGCTGCAGGAGGTGCATTGGCGATCATGGAGTTCATAC
CAGAGAAATACAAGCATATGATTGGAGGTCCTTCTCTTCAGGTTGATGTGCACACTGGAG
CTATTGCTGAAACGATCTTAAGTTTTGGTATTACCTTTGCGGTTTTGCTAATTATCCTCA
GGGGTCCACGGAGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGA
CGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTG
CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGC
AGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCGGGCAAAA
   
Sequence Name 21-K115695-SALK_069427C-R210
GenBank Accession LN484888 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K115695-SALK_069427C-R210
CATTTGTTACACCACTCCGTGGACCCCTGAGGATAATTAGCAAAACCGCAAAGGTAATAC
CAAAACTTAAGATCGTTTCAGCAATAGCTCCAGTGTGCACATCAACCTGAAGAGAAGGAC
CTCCAATCATATGCTTGTATTTCTCTGGTATGAACTCCATGATCGCCAATGCACCTCCTG
CAGCACCAATTGCCTGGAATAACAGATATAATATATCACATCTCCTTTCAAACATTATCC
CGTCGAAATCAAGAAACAGTGGCAAAAAAGGGATCATGAAGACTATTCCTACCAAAGGCA
ATAAAGTCATCTGGCTTGATTCAACTCTCAAAAAGAGTACAAATTACTCCTTGCTAAATC
AGGTTACCAAATTTAACAGTCTTTCTATCTATTTATGTTATTGTAGTACACAGAAACACA
TGGACACGCAAGAAACTCTGAAANAGGCT

Last Updated on 06.12.2018 16:11