Sequences from confirmation amplicon


Confirmation sequences for line SALK_069496c and hit At5g53360 are shown.
Line ID SALK_069496c
Locus At5g53360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21648924
Primers used for PCR 04lu - LBb3, show primer details
   
Sequence Name 60-K114145-SALK_069496C-R210
GenBank Accession LN485747 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K114145-SALK_069496C-R210
TTTGTTGACCAAACATTGAAACACCTACAAAACAATATAACAAAGAACCCCCTCAGAATC
TCAGACAACTGAAGAACTCGTAAATCATAAACAAGAACAATGCTAACCGTTAGCATCCAA
GTAGCGTTCTCTACTTCGCGTGGATTAGATTTAACATAACGATGATTAAACGTACATCCA
GTATGCATATCGACTTTATGATCATCTCTTAAATGAGCAACAAGGAAAGTAATGTCACCT
ACAGCAGCACACTCCGAGCCAGCATATGGACAACTATAAGGTCTAAAGTTACATTGAGAC
TCATGCTTTAGTTTACTGTAATAAGGGAAAATCCCAAGGCATCCAAGATTGTAATACTTG
CACGGTAACTCAAGCGACTCAGCTACTTTCTCAAGAGCGATTATGCACTCTTGATTTACA
GGTTGAACATAAAGTATGTCCATTATGACACTACAAAAGAAGAAACTTTTTCACTCATAC
ATATATCAAAACATCCATAAACCCTCAAGAAACTCAATAGTAACATACCATAGCAATGAT
AAACAAATCAGATCATTTGAAGATAACGATCATAAAAATTGGAACTTTATATTCGTAAGA
AGCAATAAGATCTTTCTCAACCCTCCNATGTTAGGAGATGATGAGATACGAACCTGATGG
ATTGGTGGGTACATTGAATTGGTGCAGACAGGGCATTCAAGGAGCTCGTAAACGCTTGTA
GCCGGAGAGATTACCACGGTGGCTCCTCCCTGAGACTTGGTTGATGAAATGGGGTTTTTT
   
Sequence Name 71-K114146-SALK_069496C-04lu
GenBank Accession LN486035 [GenBank]
Sequencing primer gene specific primer, 04lu: 5'-TCAAAAACACCAATTCTCATCAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K114146-SALK_069496C-04lu
TATCTCTCCGGCTACAGCGTTTACGAGCTCCTTGAATGCCCTGTCTGCACCAATTCAATG
TACCCACCAATCCATCAGGTTCGTATCTCATCATCTCCTAACATTGGAGGGTTGAGAAAG
ATCTTATTGCTTCTTACGAATATAAAGTTCCAATTTTTATGATCGTTATCTTCAAATGAT
CTGATTTGTTTATCATTGCTATGGTATGTTACTATTGAGTTTCTTGAGGGTTTATGGATG
TTTTGATATATGTATGAGTGAAAAAGTTTCTTCTTTTGTAGTGTCATAATGGACATACTT
TATGTTCAACCTGTAAATCAAGAGTGCATAATCGCTCTTGAGAAAGTAGCTGAGTCGCTT
GAGTTACCGTGCAAGTATTACAATCTTGGATGCCTTGGGATTTTCCCTTATTACAGTAAA
CTAAAGCATGAGTCTCAATGTAACTTTAGACCTTATAGTTGTCCATATGCTGGCTCGGAG
TGTGCTGCTGTAGGTGACATTACTTTCCTTGTTGCTCATTTAAGAGATGATCATAAAGTC
GATATGCATACTGGATGTACGTTTAATCATCGTTATGTTAAATCTAATCCACGCGAAGTA
GAGAACGCTACTTGGATGCTAACGGTTAGCATTGTTCTTGTTTATGATTTACGAGTTCTT
CAGTTGTCTGAGATTCTGAGGGGGTTCTTTGTTATATTGTTTTGTAGGTGTTTCAATGTT
TTGGTCAACAAATTGACGCTTAGACCACTTAATAACACATTGCGGACGTTTTTAATGTAC
TGGGGTGGTTTTTCTTTTCACCANTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTG
GCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCGGCCGGCGAAAATCCTGT
TTGATGGTGGTTC

Last Updated on 06.12.2018 16:11