Sequences from confirmation amplicon


Confirmation sequences for line SALK_070821c and hit At1g56080 are shown.
Line ID SALK_070821c
Locus At1g56080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20976196
Primers used for PCR 33de - LBb3, show primer details
   
Sequence Name 32-K109819-SALK_070821C-33de
GenBank Accession LN485164 [GenBank]
Sequencing primer gene specific primer, 33de: 5'-TTCTGACATCAGCAGTCTCAGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K109819-SALK_070821C-33de
GTACATCTGATTTTGTTTTGTTTCATATATAAAAGGATGTCAAAAGAAGTATAGTGTTGA
GTAACATACAGATGGATTATCATCATTCAATGACTGCATCAATTGTCTCTTGAATGCTTC
CAACTACAAGCAAAACACTAGAGCTTGAGCTTGAAATCAAATGATTAAATTTCAACAAAA
ATCAATACCATAACATTTGAAAAGAAAGAACCTTTGCATAATCACGACCGAGTTTCTTCG
CAGTGATTGCCAGTGAATCTCGTTCTTGAGTCAGTTTCATCTTCAATCCAAAAATCCAAA
AATTCAACCAATTGAGTAAAAGACACACTTTGATGATCCAAGTCAAACCAAATTTAACAA
AACTGAGGAGAATTATTACATTTTCATCGACAACATTCTTCAAACTCGAGTCAGCTTCAT
GGTAAAGTCTTTCAAAGGAAGAGACTCTGTCTTCAAGTTCATGAACAAGCCGATCCTTCT
CAAGAAGCTTCTGTCTCAAGCCGGAGACTTGAGATTCCAGATTCGAAACCCTTGAAGCGA
TNGCCATGGAAGTGATCTTCCTGGCGAGGTCGAGTTGATCATAANGGTCGGTGGGAATCA
CGGCGAGNATCTCGTCGGAGAAGTTGAAGTCA
   
Sequence Name 16-K109818-SALK_070821C-R210
GenBank Accession LN484717 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K109818-SALK_070821C-R210
ACTTCACCTCTCCGACGAGATTCTCGCCGTGATTCCCACCGACCCTTATGATCAACTCGA
CCTCGCCAGGAAGATCACTTCCATGGCTATCGCTTCAAGGGTTTCGAATCTGGAATCTCA
AGTCTCCGGCTTGAGACAGAAGCTTCTTGAGAAGGATCGGCTTGTTCATGAACTTGAAGA
CAGAGTCTCTTCCTTTGAAAGACTTTACCATGAAGCTGACTCGAGTTTGAAGAATGTTGT
CGATGAAAATGTAATAATTCTCCTCAGTTTTGTTAAATTTGGTTTGACTTGGATCATCAA
AGTGTGTCTTTTACTCAATTGGTTGAATTTTTGGATTTTTGGATTGAAGATGAAACTGAC
TCAAGAACGAGATTCACTGGCAATCACTGCGAAGAAACTCGGTCGTGATTATGCAAAGGT
TCTTTCTTTTCAAATGTTATGGTATTGATTTTTGTTGAAATTTAATCATTTGATTTCAAG
CTCAAGCTCTAGTGTTTTGCTTGTAGTTGGAAGCATTCAAGAGACAATTGATGCAGTCAT
TGAATGATGATAATCCATCTGTATGTTACTCAACACTATACTTCTTTTGACATCCTTTTA
TATATGAAACAAAACAAAATCAGATGTTACATACTCTCCTCTTGTTTTTACAGCANACTG
AGACTGCT

Last Updated on 06.12.2018 16:11