Sequences from confirmation amplicon


Confirmation sequences for line SALK_071403c and hit At3g18035 are shown.
Line ID SALK_071403c
Locus At3g18035 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6170385
Primers used for PCR BI02 - R204, show primer details
   
Sequence Name 55-K107002-SALK_071403C-R210
GenBank Accession LN485614 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K107002-SALK_071403C-R210
GCGTCATTTGTTTACCCCAATATCTCGATTTTGCAATATCTGACGTTTACTGTGCATGAT
TGTTCACTGCTCCGCCGTCAATTTAGATTGATAAAGTTTGATACTTGTGTTGCAATCATT
GTGGAATTCAAACTCAATGTCACTTTCCGCTTTTGAGCATTGTTGTCTGTTTTCGCGTAT
CCAAAATCTGACTAGTATCTAGTGTTTTTATTATATTTTCGTGTCCCTTACTTTTGGCTT
ATCCTTATCTGGTTTCTCTGTTTTATTTTAATTTTCGTTTGGATAATTAAGACCAAAATA
GTCGGTAATGTATCGCTATAGCTAGGAATAATTTTATCATCAATTAGTGTGTTAATCCGG
CTTAATGGTTTCTAAGTCATCACATAGTGTCTTCTTCTCTGTATCTTCTATTTTACTAGT
AACGTGTTCAATTGGATCTAAAGTAAGACTTCTTAATTTAACCATTAATGTCTTTCTTGA
ATATGTTATACTTTTATCCAACAATTTTATTTTTTGGTTCATGAACCTAGATTGGAACAT
CAGTCACGACTGGGACACAAGATTCTGGAGAACTCAAGAAGAAGTTTGATATTTTTGTAA
GTCTACTTGTTGACAATAAACAGTTTGGCTGTCCTTTATTTGATTAAAGTATTCGATTCT
ACTTGCTTATGTTTGGTTTTTGTATGTAAATGATTTCAGCAAGAGAAAGTGAAAGAAATT
GTGAAGGTGTTGAAGGATGGATTTACAAGTG
   
Sequence Name 63-K107003-SALK_071403C-BI02
GenBank Accession LN485810 [GenBank]
Sequencing primer gene specific primer, BI02: 5'-CACTTGTAACTCCATCCTTCAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K107003-SALK_071403C-BI02
TACATGCGGACCCAACACATAAGCAAGTAGAATCGAATACTTTAATCAAATAAAGGACAG
CCAAACTGTTTATTGTCAACAAGTAGACTTACAAAAATATCAAACTTCTTCTTGAGTTCT
CCAGAATCTTGTGTCCCAGTCGTGACTGATGTTCCAATCTAGGTTCATGAACCAAAAAAT
AAAATTGTTGGATAAAAGTATAACATATTCAAGAAAGACATTAATGGTTAAATTAAGAAG
TCTTACTTTAGATCCAATTGAACACGTTACTAGTAAAATAGAAGATACAGAGAAGAAGAC
ACTATGTGATGACTTAGAAACCATTAAGCCGGATTAACACACTAATTGATGATAAAATTA
TTCCTAGCTATAGCGATACATTACCGACTATTTTGGTCTTAATTATCCAAACGAAAATTA
AAATAAAACAGAGAAACCAGATAAGGATAAGCCAAAAGTAAGGGACACGAAAATATAATA
AAAACACTAGATACTAGTCAGATTTTGGATACGCGAAAACAGACAACAATGCTCAAAAGC
GGAAAGTGACATTGAGTTTGAATTCCACAATGATTGCAACACAAGTATCAAACTTTATCA
ATCTAAATTGACGGCGGAGCAGTGAACAATCATGCACAGTAAACGTCAGATATTGCAAAA
TCGAGATATTGTGGTGTAAACAAATTGACGCTAAGACAACTTAATAACACATTGCGGACG
TTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCC
CTTCACCGCCTGGCCCTGAGAGAGTTGCAGCA
Primers used for PCR 77xo - LBb3, show primer details
   
Sequence Name 72-K119300-SALK_071403C-77xo
GenBank Accession LN486069 [GenBank]
Sequencing primer gene specific primer, 77xo: 5'-TCTCCATTCAAGTAACAACGATCC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K119300-SALK_071403C-77xo
CGAGGTCGTGGTCGTCCTCCTAAGCCTAAACCTGAATCTCAACCACAACCACTACAGCAA
CTTCCACCGACCAATCAAGTCCAGGCTAACGGACAGCCAATCTGGGAACAGCAGCAAGTT
CAATCACCTGTTCCGGTTCCGACTCCGGTTACAGAGTCGGCGAAGAGAGGACCTGGTCGT
CCAAGGAAGAACGGTTCTGCTGCTCCTGCTACTGCACCAATCGTTCAAGCTTCGGTTATG
GCTGGAATTATGAAACGTAGAGGTAGACCACCGGGTCGTCGAGCTGCTGGGAGACAGAGG
AAGCCCAAATCCGTTTCTTCTACTGCCTCTGTGTATCCTTATGTTGCTAATGGTGCTAGA
CGCAGAGGAAGGCCTAGGAGAGTTGTTGACCCTAGCAGTATTGTTAGTGTTGCTCCAGTA
GGTGGTGAAAATGTGGCAGCGGTTGCGCCAGGGATGAAGCGTGGACGTGGACGACCACCT
AAGATTGGTGGTGTTATCAGTAGGCTTATTATGAAGCCTAAGAGAGGACGAGGACGTCCT
GTAGGTAGACCCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAAC
ACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAA
CAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGT
TTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCGGGCAAA
   
Sequence Name 24-K119299-SALK_071403C-R210
GenBank Accession LN484992 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K119299-SALK_071403C-R210
GTCATTTGTTACCCACAATATATCCTGGGTCTACCTACAGGACGTCCTCGTCCTCTCTTA
GGCTTCATAATAAGCCTACTGATAACACCACCAATCTTAGGTGGTCGTCCACGTCCACGC
TTCATCCCTGGCGCAACCGCTGCCACATTTTCACCACCTACTGGAGCAACACTAACAATA
CTGCTAGGGTCAACAACTCTCCTAGGCCTTCCTCTGCGTCTAGCACCATTAGCAACATAA
GGATACACAGAGGCAGTAGAAGAAACGGATTTGGGCTTCCTCTGTCTCCCAGCAGCTCGA
CGACCCGGTGGTCTACCTCTACGTTTCATAATTCCAGCCATAACCGAAGCTTGAACGATT
GGTGCAGTAGCAGGAGCAGCAGAACCGTTCTTCCTTGGACGACCAGGTCCTCTCTTCGCC
GACTCTGTAACCGGAGTCGGAACCGGAACAGGTGATTGAACTTGCTGCTGTTCCCAGATT
GGCTGTCCGTTAGCCTGGACTTGATTGGTCGGTGGAAGTTGCTGTAGTGGTTGTGGTTGA
GATTCAGGTTTAGGCTTAGGAGGACGACCACGACCTCGTTTCAGAGGCTGAGAAGCAGAG
CCAGAAGCCATGGGATCGTTGTTACTTGAATGGAGA

Last Updated on 06.12.2018 16:11