Sequences from confirmation amplicon


Confirmation sequences for line SALK_072332c and hit At5g05850 are shown.
Line ID SALK_072332c
Locus At5g05850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1763424
Primers used for PCR 89ns - LBb3, show primer details
   
Sequence Name 33-K115696-SALK_072332C-89ns
GenBank Accession LN485188 [GenBank]
Sequencing primer gene specific primer, 89ns: 5'-CTTCATATTCGCCATCTTCTCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K115696-SALK_072332C-89ns
TATACATTTAACCGCATCCACACCTTGTTTCACGACTTCATCAGGCGGGACAACAANGCG
GGTTTTGGTCCAAGTTCAGTTTGGTCAAGTTCACGAGCGTGCCAAAAGCGTCTGGAAGAG
AATGGATCTGGTTGTTGCTCAAGTCGAGTTCTTGGAGGCTTATGAGATCTCCGAATGAAG
CAGGGAGATCTTGGAGATCGCTGAAGTTACTGCTCAGGTTAAGGTACTCGAGGTTCGTGA
GCAGCCCGAAAGAATTTGGGAGACCATTGAGTTCGTTGAAGTGTGCATCGAGGTAGCGTA
AGGATCTCATTTCACCAATGGAAGTTGGTAATGATCTGATTTTGTTGAGATGGATCAAGA
GCTTCTCTAGTTTCACTAGCTCGAATCCGATGTTTGTTGGTAAGTAGGTGAGATTGTTGT
AGCTCGCATCCAAGACAACCAACGATCTTTAAATAAAAAAAAAAACAGAAGTAAGAAAAA
AACAGAGGAAACAGAGTCAACCTTTAAAGATAAAAAACACTTACCCACAATGGCAAATGG
AATCTGATATATTGTGGTGTAAACAAATTGACGCTTAAACAACTTAATAACACATTGCGG
ACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATT
GCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAG
CAGGCGAAAATCCTGTTTGATGGTGGTTC
   
Sequence Name 22-K115695-SALK_072332C-R210
GenBank Accession LN484921 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K115695-SALK_072332C-R210
CATTTGTTTACCCCAATATATCAGATTCCATCTGCCATTGTGGGTAAGTGTTTTTTATCT
TTAAAGGTTGACTCTGTTTCCTCTGTTTTTTTCTTACTTCTGTTTTTTTTTTTATTTAAA
AATCGTTGGTTGTCTTGGATGCAAGCTACAACAATCTCACCTACTTACCAACAAACATCG
GATTCGAGCTAGTGAAACTAAAGAAGCTCTTGATCCATCTCAACAAAATCAAATCATTAC
CAACTTCCATTGGTGAAATGAAATCCTTACCCTACCTCGATGCACACTTCAACAAACTCA
AGGGTCTCCCAAATTCTTTCGGGCTGCTCACAAACCTCGAGTACCTTAACCTGAGCAGTA
ACTTCAGCGATCTCCAAAATCTCCCTGCTTCATTCGAAAATCTCAAAACCCTCCAAAAAC
TCGACTTGAGCAACAACCAAATCCATTCTCTTCCAAACCCTTTTGGCACGCTCGTGAACT
TGACCAAACTGAACTTGGACCAAAACCCGCTTGTTGTCCCCCCTGATAAATTCGTGAAAC
AAGGTGTGGATGCGGTTAAAATGTATATGGGTAAAAAATGGGTCACCATGTTGAAAAAGG
AAAAAAAAATGGCAAAA

Last Updated on 06.12.2018 16:11