Sequences from confirmation amplicon


Confirmation sequences for line SALK_073252c and hit At3g04520 are shown.
Line ID SALK_073252c
Locus At3g04520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1217609
Primers used for PCR AW25 - LBb3, show primer details
   
Sequence Name 15-K111624-SALK_073252C-AW25
GenBank Accession LN484694 [GenBank]
Sequencing primer gene specific primer, AW25: 5'-TTTTATTTTTCAATCCGTGATTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K111624-SALK_073252C-AW25
ATATCGGATATAAGAATAATTATTGGGTTATAATTGGTTATTTATATGTATAAGTGAATT
TGATGAAAGAGTTTTCTACTTCTTGGTTAAAGGTTTTCATTTGTAAACCAAGAAAATCGA
TTCAAATACAACCTTATTCTGAATGAAGATTCAAATACAACCTTACACATGTCCAACGAG
TGAAATTACTTAAAATAATAACGCCTTCTCTTCTCTTCACGAGTGAAATATTTTCTGAAA
TATATCGAAAGCAATTAATCAATGAATGAATAAAGCGTAGCAAAGAAATTAAAAATATCA
AAGCACATATCAGTAGATGAAAGCAAACTACATACCTCAAAGCAAGAGAGTACATATTCC
ACGTCGACGTCTGAGATTTGGTGGTGTAGAACAATCCTGATTCTGTATTAAGAGACCATA
AACTCCAAGAACAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAAC
ACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAA
CAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGT
TTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAATCGGGCAAAA
   
Sequence Name 07-K111623-SALK_073252C-R210
GenBank Accession LN484457 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K111623-SALK_073252C-R210
TTTGTTTACCACAATATATCCTGTTCTTGGAGTTTATGGTCTCTTAATACAGAATCAGGA
TTGTTCTACACCACCAAATCTCAGACGTCGACGTGGAATATGTACTCTCTTGCTTTGAGG
TATGTAGTTTGCTTTCATCTACTGATATGTGCTTTGATATTTTTAATTTCTTTGCTACGC
TTTATTCATTCATTGATTAATTGCTTTCGATATATTTCAGAAAATATTTCACTCGTGAAG
AGAAGAGAAGGCGTTATTATTTTAAGTAATTTCACTCGTTGGACATGTGTAAGGTTGTAT
TTGAATCTTCATTCAGAATAAGGTTGTATTTGAATCGATTTTCTTGGTTTACAAATGAAA
ACCTTTAACCAAGAAGTAGAAAACTCTTTCATCAAATTCACTTATACATATAAATAACCA
ATTATAACCCAATAATTTATTCTTTATAATCCGATTATTCCTCATGTGCTAATAGTTTGT
GTCCGGAATCACGGATTGAAAAATAAA

Last Updated on 06.12.2018 16:11