Sequences from confirmation amplicon


Confirmation sequences for line SALK_073367c and hit At3g21100 are shown.
Line ID SALK_073367c
Locus At3g21100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7400859
Primers used for PCR 51cy - LBb3, show primer details
   
Sequence Name 07-K109680-SALK_073367C-51cy
GenBank Accession LN484454 [GenBank]
Sequencing primer gene specific primer, 51cy: 5'-TAGCCATACCCTTTGTTAAGATGG-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K109680-SALK_073367C-51cy
GTATCACTCANAAAGCTCGGGTTGCTTCAGACTGATGTCCTGAATCACCATTACCTGCTA
AGAGGTAACACGGAAGAGGGCAAAAGTAAATAAAAGCTATATAAAGTCAAAGTTCGATGT
AGGCAAGATAGACTTGATCACCATTACCTTCGAAGACTTCATCACTGGTGCTTTCTGATC
GAAAGAGCATGCTTTGATTGACACGAGGGGAAAAATGGACGGGGGATCCAATGGAAAGAC
TACGCTGGTGATGGAGAAACGATTCACTGTCCATGTCGGGCAACTGCAGACTCAAAAATC
TTCTCCTTTGGAATTCTATTGCTTGTTGCAGGTCAGCTTGCTCAGCTTTTCTCCTCATCA
TCTCCTGTGTGTTAGAAAACATCCTTGGTGCTGGTAGCAAACAAAACAAGACTGGTCTCA
GAACCCATAACAAAAAAATCGAAAGAAGAGGTTGAGCTCTGATGAAACCATATCCACAAG
GGGAATCAAGCATAGGAAATAAATTCAACTAACCGAGATGAGAATCAAAGAGATCCCTGG
AATCCATTCCAGATGGACTTGAACCAGGAGAAAAGTTTCCTCTCTCCATCTGCTGCAGCA
GTTGTTGCTGCTGCCTCCTGCTATGGTTCAATAAGGATTATTATAAGAAAAAGGGAACAC
AAAAAGAGAAGAAGAATATGGGAAGGAGAGACAACTTACAGTTGATAACACATTGACGCT
TAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCA
CCAGTGAGACGGGCAACAGCTG
   
Sequence Name 23-K109679-SALK_073367C-R210
GenBank Accession LN484942 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K109679-SALK_073367C-R210
TGTTATCACTGTAAGTTGTCTCTCCTTCCATATTCTTCTTCTCTTTTTGTGTTCCCTTTT
TCTTATAATAATCCTTATTGAACCATAGCAGGAGGCAGCAGCAACAACTGCTGCAGCAGA
TGGAGAGAGGAAACTTTTCTCCTGGTTCAAGTCCATCTGGAATGGATTCCAGGGATCTCT
TTGATTCTCATCTCGGTTAGTTGAATTTATTTCCTATGCTTGATTCCCCTTGTGGATATG
GTTTCATCAGAGCTCAACCTCTTCTTTCGATTTTTTTGTTATGGGTTCTGAGACCAGTCT
TGTTTTGTTTGCTACCAGCACCAAGGATGTTTTCTAACACACAGGAGATGATGAGGAGAA
AAGCTGAGCAAGCTGACCTGCAACAAGCAATAGAATTCCAAAGGAGAAGATTTTTGAGTC
TGCAGTTGCCCGACATGGACAGTGAATCGTTTCTCCATCACCAGCGTAGTCTTTCCATTG
GATCCCCCGTCCATTTTTCCCCTCGTGTCAATCAAAGCATGCTCTTTCGATCAGAAAGCA
CCAGTGATGAAGTCTTCGAAGGTAATGGTGATCAAGTCTATCTTGCCTACATCGAACTTT
GACTTTATATAGCTTTTATTTACTTTTGCCCTCTTCCGTGTTACCTCTTAGCAGGTAATG
GTG

Last Updated on 06.12.2018 16:11