Sequences from confirmation amplicon


Confirmation sequences for line SALK_073421 and hit At2g22450 are shown.
Line ID SALK_073421
Locus At2g22450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9531159
Primers used for PCR BI68 - R204, show primer details
   
Sequence Name 51-K106282-SALK_073421C-R210
GenBank Accession LN485522 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K106282-SALK_073421C-R210
GCGTCATTTGTGTTATTGAAGATATTCGACATGGCAAGGTTTGTAATAGTAGCATCGTGT
CTGCTTACTGTCTTATTAGAAGGGGAAGGTCTTAAGTGAGAAAGCTTTGCGTTTTTCTTG
CAGATGGTAGTTGTTGTAGATGATGAAGATAGAGAAAACGAAGGAGATTTGATAATGGCG
GCATCTTTGGCTACACCTGAAGCTATGGCTTTTGTTGTAAAGCATGGGACTGGAATCGTG
TGTGTGAGCATGAAAGGCGAAGACTTGGAGAGGTTAGAGCTTCCTTTAATGGTAACGCGT
AAGGATAACGAGGAAAAACTCCGCACGGCCTTCACGGTTTCAGTGGTGAGTAAAAGTATG
TTTTTCTTTGGTGTTGAGAAAACCACAAGACTCTTAGTGGTGTGTGATGAAAAGAATGTT
GAAAATATTTGCCAGGACGCAAAAAAGGGAACATCCACAGGTGTCTCAGCTCGTGATAGG
GCGCAAACAATATTAACCCTTGCATCAAAAGATTCAAAACCTGAAGATTTCAATCGTCCT
GGTCATATTTTCCCTTTGA
   
Sequence Name 67-K106283-SALK_073421C-BI68
GenBank Accession LN485917 [GenBank]
Sequencing primer gene specific primer, BI68: 5'-TCAAAGGAAAAATATGACCAGGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K106283-SALK_073421C-BI68
GATGCAGGGTTATATTGTTTGCGCCCTATCACGAGCTGAGACACCTGTGGATGTTCCCTT
TTTTGCGTCCTGGCAAATATTTTCAACATTCTTTTCATCACACACCACTAAGAGTCTTGT
GGTTTTCTCAACACCAAAGAAAAACATACTTTTACTCACCACTGAAACCGTGAAGGCCGT
GCGGAGTTTTTCCTCGTTATCCTTACGCGTTACCATTAAAGGAAGCTCTAACCTCTCCAA
GTCTTCGCCTTTCATGCTCACACACACGATTCCAGTCCCATGCTTTACAACAAAAGCCAT
AGCTTCAGGTGTAGCCAAAGATGCCGCCATTATCAAATCTCCTTCGTTTTCTCTATCTTC
ATCATCTACAACAACTACCATCTGCAAGAAAAACGCAAAGCTTTCTCACTTAAGACCTTC
CCCTTCTAATAAGACAGTAAGCAGACACGATGCTACTATTACAAACCTTGCCATGTCGAA
TATCTTCAATAACACAAATTGACGCTNAGACAACTTAATAACACATTGCGGACGTTTTTA
ATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCAC
CGCCTGGCCCTGAGAGAGTTGCAGCA
Primers used for PCR BI69 - LBb3, show primer details
   
Sequence Name 12-K119293-SALK_073421C-R210
GenBank Accession LN484630 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K119293-SALK_073421C-R210
GTCATTTGTTTACCCACAATATATCCTTGACGCAAATCCATCAGTAGGTCGATCTAAATC
AAACTCTTCATCCTCAACTTCAGGAGAATCCATTGTTGTAGGAATCATTTCAGCTGCTAA
TGTTCCAAACGAAACCAAATCTGGCTGTAACTCAATCCCCATTGATGATCCATTACTCTT
AGAAGAAGAGAACACATCTCCTTCCTCAGGTATCACTCTCATTCTAACATTAAACTTCTT
CGATATGATCCTAGGATACACCAAGCTCGTTCCAAATGGTTTTGTTCCCTTGACAACATC
ACTGAAAAACCCATGAAGACAACATAACCATCAAAACCTAAATACATAGATATGTGTTTC
AATTGAATCCAAAATCGAATCATAAGCAAACGTAATTTCAAATTCCGAATGATAAACAGA
TCTAATCAAAAACTATCAATTCAAATTCAGTGAAACAGAGAGAAATACAATTCAATTTGA
TTACCGGGAAGGCAAAAGATGCGTCGAATCGCACCGGAGAGTAAGCGACGCCATTTTCAC
CGGAGTTTTGAAGAGAGAGAACGACGA

Last Updated on 06.12.2018 16:11