Sequences from confirmation amplicon


Confirmation sequences for line SALK_073422 and hit At1g30810 are shown.
Line ID SALK_073422
Locus At1g30810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10938356
Primers used for PCR 59cs - LBb3, show primer details
   
Sequence Name 89-K109033-SALK_073422C-59cs
GenBank Accession LN486326 [GenBank]
Sequencing primer gene specific primer, 59cs: 5'-TTCTGCATCCACAGTACATTTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K109033-SALK_073422C-59cs
CTCTGTTCGACTCACATTCCGGCGACTTTTTCCGGCGAAGCAACCGTCAAGATTCCACTA
ACGGCGTGAAATATCTCTTTTTTGTCTTTACAAGATTTTTTAGACTCAAAGTCTAATTTT
TTTCAAGATTTTGTGAAACTCTGTTACATAATGTAAAGTTTTTCACATTAAGCAATAGCA
AATTTTCTTGATTTTTGAAAGAAGAATTTCTATTATTATGACTTTTCTTTTCTAGTTTAC
ACTTCACTAACGCTAAGGTTAGGTTATTTGTTCTTGCTATGATAAATAAGAACAAAAGAT
TATGAATAATAAATGCCCCCAAAACAAAAAAAAAAAATCAAAAAAAAATTTCTTCTCTCT
ATCTATCAAAAAAAAATGCCATGGTGGTTCCGGTGGTTGTGGTGGGCCCCGAAGTGGGAA
CAGTCTTTTTTGTTTCCATCAAATCTACTCCAAAAAGTTTCCTTTTGGTTAAACTTGCGG
AACATTTTGAT
   
Sequence Name 73-K109032-SALK_073422C-R210
GenBank Accession LN486073 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K109032-SALK_073422C-R210
CATTTGTTACCCACAATATATTAGAATAGCTGTGGGCTTCAGTCACCACACCTTTAACCT
TGTTCTTCTTCATTAAAAAGCTATTCATGATGTTAACTCTTACACAACTTAACGCTTCTC
TACAGGCCATTGAAGCTCTTGACCCAAACCATCGACTAGTTGAGTACTGGAACCACAAGA
ACCAAACTTCATCAGACTCAAAAGATCACTTCATATCATCAAACTGTTCCGCAAGTTTAA
CCAAAGGAAAACTTTTCGGAGTAGATTTGATGTAAACAAAAAAGACTGTACCAACTCCGA
GACCAACAACAACAACAGAAACAACAATGACATATCTTTTAGATAGATAGAGAGAAGAAA
TTTCTTTCTGATTTTTTTTTTTTGTTTGGGGGGCATTTATAATCCAAAACCTTTGGTCCT
TATTTATCAAAGCAGGAACAAATAACCTACCCTTGGCGTTGGGGAAGGGTAAACTAAAAA
AAAAAAGTCTTAATAATAAAAATTCTTCTTTCAAAAATCAAAAAAATTTGCTTTTGCTTA
AGGGGAAAAACTTTCCATTTTGTAAAAAAGTTTCCCAAAATCTTGAAAAAAATTAAACTT
TGAGTTTAAAAAATCTTGTAAAAACAAAAAAAAAATATTTCACCCCGTTAGGGGAATTTT
GACGGTTACTTCCCCGAAAAAATTCCCCG

Last Updated on 06.12.2018 16:11