Sequences from confirmation amplicon


Confirmation sequences for line SALK_073618 and hit At2g02970 are shown.
Line ID SALK_073618
Locus At2g02970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:867121
Primers used for PCR 26dh - LBb3, show primer details
   
Sequence Name 64-K111283-SALK_073618C-26dh
GenBank Accession LN485844 [GenBank]
Sequencing primer gene specific primer, 26dh: 5'-GAGACTGAAGTTAGGTTAATGGCG-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K111283-SALK_073618C-26dh
AAAGATTCTGGGAGTTGCAAGAAGAGTTCTTAAATCTTCTGGGTTCTTGTTTCGAGATGA
ATGGGCTTCTGTTATCTCTGGTGAGTTTTCAAAATCTTCTCTTTTGTTCAAAAGTGAGTC
TTTTTATCTAATGCCATTGGAGTTGTATCAGTTTCTGTCTCGCTCTTTTTGATTTGAGAG
TTTGTTGCTCCTTTATTGTTACTAGCTAATCATTGGATACTAAATTTGCCAGGATCTGAC
GAGGGTGTATATGCTTGGGTTGTTGCAAACTTTGCTCTCGGTTCACTTGGAGGTGATCCT
CTTAAAACAACCGGAATTGTTGAACTTGGCGGGGCTTCTGCTTGACGCTTGCAAACAAAT
TGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTT
CTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGT
TGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTT
CCGA
   
Sequence Name 48-K111282-SALK_073618C-R210
GenBank Accession LN485456 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K111282-SALK_073618C-R210
TTTGTTTGCAGCGTCAGCAGAAGCCCCGCCAAGTTCACAATTCCGGTTGTTTTAAGAGGA
TCACCTCCAAGTGAACCGAGAGCAAAGTTTGCAACAACCCAAGCATATACACCCTCGTCA
GATCCTGGCAAATTTAGTATCCAATGATTAGCTAGTAACAATAAAGGAGCAACAAACTCT
CAAATCAAAAAGAGCGAGACAGAAACTGATACAACTCCAATGGCATTAGATAAAAAGACT
CACTTTTGAACAAAAGAGAAGATTTTGAAAACTCACCAGAGATAACAGAAGCCCATTCAT
CTCGAAACAAGAACCCAGAAGATTTAAGAACTCTTCTTGCAACTCCCAGAATCTTTTCCT
GAACAGGTAATTCAAGCAATCTCATTCCCGCCGTCGCCATTAACCTAATNTCAGTCT

Last Updated on 06.12.2018 16:11