Sequences from confirmation amplicon


Confirmation sequences for line SALK_073785 and hit At2g30790 are shown.
Line ID SALK_073785
Locus At2g30790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13120028
Primers used for PCR 04bc - R204, show primer details
   
Sequence Name 03-K107118-SALK_073785C-R210
GenBank Accession LN484325 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K107118-SALK_073785C-R210
GTCATTTGTTACACCACAATATATCCTGGCAATATATCTTCATCTTCAACGTCGTTTCGT
CATGTGTCCCTCTCGAGACCCGTCCATTTAGTATGTAGAGCTCAACAGTCTCAGGAAAAC
AATAACTCTGCTGTTTTCCGCCGTCTTGCCCTTACCTTACTCGTTGGTGCTGCAGCCATC
GGATCCAAAGTCTCTCCTGCGCCGCCTATGGTGAAGCCGGTATGATCCCACTTTCTCTAT
ATACATTTTGAGCATATATCAATGAGAATCATGAAGCAAATCTTGATTGTTGTTTTGCAG
CTAATGTGTTTGGTAAGCCAAAGAAGAACACAGATTTCCTGCCATACACAGGAGAAGGAT
TCAAAATCCAGATCCCATCAAAGTGGAACCCAAGTAAAGAAATTGAGTATCCAGGACAAG
TACTAAGATTCGAGGACAACTTTGACGCCACCAGCAATGTCTCTGTCATGATCACTCCTA
CAGACAAGAAATCCATCACTGATTACGGTTCTCCTGAACAGTTCCTCTCTCAGGTTTCAC
TCTGTTTTTAAAGCAAAAACGGTTCAATCAGACGATAATCACCACAACACAGCTGATGAT
AGGTTTCTGGTTTCTGCAGGTGAATTATCTCCTCGGGAAGCAAGCTTACGTCGGCGAAAC
AGCCTCTGAGGTAATTTCCTTTTCTAGTTGGTGAAGGAGTTTTTGGGTTCAAACAAGAAC
CAATACCTTAAAGAGTGTGACTGATCATTTTTGCAGGGAGGGTTTGATGCAAATGCAGTA
GCAACTGCAAACATTTTGGAAACATCTACACAA
   
Sequence Name 19-K107119-SALK_073785C-04bc
GenBank Accession LN484803 [GenBank]
Sequencing primer gene specific primer, 04bc: 5'-AGTATTCTTTCCCTCCGATTTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K107119-SALK_073785C-04bc
TTTAAGGTATTGGTTCTTGTTTGAACCCAAAAACTCCTTCACCAACTAGAAAAGGAAATT
ACCTCAGAGGCTGTTTCGCCGACGTAAGCTTGCTTCCCGAGGAGATAATTCACCTGCAGA
AACCAGAAACCTATCATCAGCTGTGTTGTGGTGATTATCGTCTGATTGAACCGTTTTTGC
TTTAAAAACAGAGTGAAACCTGAGAGAGGAACTGTTCAGGAGAACCGTAATCAGTGATGG
ATTTCTTGTCTGTAGGAGTGATCATGACAGAGACATTGCTGGTGGCGTCAAAGTTGTCCT
CGAATCTTAGTACTTGTCCTGGATACTCAATTTCTTTACTTGGGTTCCACTTTGATGGGA
TCTGGATTTTGAATCCTTCTCCTGTGTATGGCAGGAAATCTGTGTTCTTCTTTGGCTTAC
CAAACACATTAGCTGCAAAACAACAATCAAGATTTGCTTCATGATTCTCATTGATATATG
CTCAAAATGTATATAGAGAAAGTGGGATCATACCGGCTTCACCATAGGCGGCGCAGGAGA
GACTTTGGATCCGATGGCTGCAGCACCAACGAGTAAGGTAAGGGCAAGACGGCGGAAAAC
AGCAGAGTTATTGTTTTCCTGAGACTGTTGAGCTCTACATACTAAATGGACGGGTCTCGA
GAGGGACACATGACGAAACGACGATGAAGATGAAGATATATTGCCAGGATATATTGTGGT
GTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGG
GGTGGTTTTTCTTTTCACCAGTGAGACGGGCACAGGCT

Last Updated on 06.12.2018 16:11