Sequences from confirmation amplicon


Confirmation sequences for line SALK_074025c and hit At1g10340 are shown.
Line ID SALK_074025c
Locus At1g10340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3392194
Primers used for PCR 07dx - LBb3, show primer details
   
Sequence Name 78-K117830-SALK_074025C-07dx
GenBank Accession LN486198 [GenBank]
Sequencing primer gene specific primer, 07dx: 5'-TACTCACAGGAGCATCAAAAGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K117830-SALK_074025C-07dx
CCCTTTCATCGGTTTGCTGTAGAAGAATTTGGCTGTTAATTCCCAAACTCTCTGCCATAA
AAACAAAGGCATCCATATTTTTGTTTCGAGCAGCGAGATGAAAGACTGTCTCTTTCGACG
GCGTGATTGAGCTGAAAGACAATGGAACCTTGTCCAAGAACTCCTCCAGGATCACAACCG
AGCCTCTGAGGACCGCAAGATGCAGAGGTGATAAACCATTGGGGTTAAGTGCTTCTTCTA
ATCCTTGATCGAGCCCTAACAATATAGTTGTCAGTTCAAAGTCTCCCTTATCACACGCAT
GATGCAGTAGCGTTGATTGTGAGCCGTCTTCAACCACCCAAGCTTCTTCCCTAGCTAGGT
CTGGGAATCTCTCCAGTATAGTCCCTACGATACCTGCTCAAGGATCCAAACACAGTAGTA
GTGAGCTAGTGATTTTTACACAAGATTACGAAAAATGTTGAGCGCGCACTAGAAAAACTT
ACTAGTGGATCCACTTGATATGGCGAGAATGAGTTCACCGAGGCCAATTGATTGTGTCTT
TTCCGCGATGAGTCTGGCAGCCTCTATGGAATTGCTACGGCAAGCCAAGTGGAGTGGTGT
GTGGTTGTTGATATTGCGTGCAGAACACACTTCCAATAACACATTGACGCTTAGACAACT
TAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGA
CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCA
CGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 61-K117829-SALK_074025C-R210
GenBank Accession LN485775 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K117829-SALK_074025C-R210
TGGAGTGTGTTCTGCACGCAATATCAACAACCACACACCACTCCACTTGGCTTGCCGTAG
CAATTCCATAGAGGCTGCCAGACTCATCGCGGAAAAGACACAATCAATTGGCCTCGGTGA
ACTCATTCTCGCCATATCAAGTGGATCCACTAGTAAGTTTTTCTAGTGCGCGCTCAACAT
TTTTCGTAATCTTGTGTAAAAATCACTAGCTCACTACTACTGTGTTTGGATCCTTGAGCA
GGTATCGTAGGGACTATACTGGAGAGATTCCCAGACCTAGCTAGGGAAGAAGCTTGGGTG
GTTGAAGACGGCTCACAATCAACGCTACTGCATCATGCGTGTGATAAGGGAGACTTTGAA
CTGACAACTATATTGTTAGGGCTCGATCAAGGATTAGAAGAAGCACTTAACCCCAATGGT
TTATCACCTCTGCATCTTGCGGTCCTCAGAGGCTCGGTTGTGATCCTGGAGGAGTTCTTG
GACAAGGTTCCATTGTCTTTCAGCTCAATCACGCCGTCGAAAGAGACAGTCTTTCATCTC
GCTGCTCGAAACAAAAATATGGATGCCTTTGTTTTTATGGCAGAGAGTTTGGGAATTAAC
AGCCAAATTCTTCTACAGCAAACCGATGAAAGTGGCAACACTGTCTTACATATTGCTGCA
TCCGTCTCTNTTGATGCTCCTGTGAGT

Last Updated on 06.12.2018 16:11