Sequences from confirmation amplicon


Confirmation sequences for line SALK_074937 and hit At3g53930 are shown.
Line ID SALK_074937
Locus At3g53930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19966782
Primers used for PCR 09bh - LBb3, show primer details
   
Sequence Name 19-K108069-SALK_074937C-09bh
GenBank Accession LN484804 [GenBank]
Sequencing primer gene specific primer, 09bh: 5'-TGTAACGGACACCAATAATTTCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K108069-SALK_074937C-09bh
ACACAGATTTTTTGTTTTGTTTAAGGTAGTAATGTTTACAATTCGAAAATATTTGAAGAT
TTGTAGTAACGACGCGTAGAAACATAAGTTCGTAGTTATTTCATAACTTTAAATTGTTAT
GATTGGTGTAGCTTGTAGCGATGACTTCATAGTTTAAGACCACTAATCATGCTGTCATTA
TGTCCACTCAAAGCCGTAATTTTAAATAAAACCATACTATTAAAATTTAATACAAATCAT
CATCATCTCCGATCGTATAAATCAGAAGCGTCATTTTACAAGTTGATTCCTTTGCTCTAA
TAATATTAGTAAAAAAAAACTTTGCATTGAATTCAATTCAAAATAAACCTTTCTTTCTTT
CTTTCTTTCTTCTCTATCATTTTATCTGAATCCGCCTCCCAAATTCAATCCTTTAATTTT
CAACTCCAAATCGATCCTTCAATCAATTTCTGAAACTTTTAGTCATCATCAGCTCAATTT
CGGATCTCCGGAGTTGTATTTTTACTCCTTTGTCGGAGGTAAAATGGCTCAGTCGTCGTT
GGTGGCGGCGGCGGGGAGAAGCGGAAGGGTTATCGGCGATTATGCGGTGGGTCGACAAAT
CGGGTCGGGGTCGTTTTCCGTGGTTTGGGAAGGGAGGCATCTTGTTCATGGCACCGTCGT
TGCTATCAAGGAGATCGCTATGGCTCGGCTTAATAAGAAGTTGCAAGAGAGTCTCATGTC
GGAGATTATCATCTTAAGGAAGATTAATCATCCTAACATCATTCGGACAAATTGACGCTT
AGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCAC
CAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGC
   
Sequence Name 30-K108070-SALK_074937C-R210
GenBank Accession LN485121 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K108070-SALK_074937C-R210
GCGTCATTTGTCGAATGATGTTAGGATGATTAATCTTCCTTAAGATGATAATCTCCGACA
TGAGACTCTCTTGCAACTTCTTATTAAGCCGAGCCATAGCGATCTCCTTGATAGCAACGA
CGGTGCCATGAACAAGATGCCTCCCTTCCCAAACCACGGAAAACGACCCCGACCCGATTT
GTCGACCCACCGCATAATCGCCGATAACCCTTCCGCTTCTCCCCGCCGCCGCCACCAACG
ACGACTGAGCCATTTTACCTCCGACAAAGGAGTAAAAATACAACTCCGGAGATCCGAAAT
TGAGCTGATGATGACTAAAAGTTTCAGAAATTGATTGAAGGATCGATTTGGAGTTGAAAA
TTAAAGGATTGAATTTGGGAGGCGGATTCAGATAAAATGATAGAGAAGAAAGAAAGAAAG
AAAGAAAGGTTTATTTTGAATTGAATTCAATGCAAAGTTTTTTTTTACTAATATTATTAG
AGCAAAGGAATCAACTTGTAAAATGACGCTTCTGATTTATACGATCGGAGATGATGATGA
TTTGTATTAAATTTTAATAGTATGGTTTTATTTAAAATTACGGCTTTGAGTGGACATAAT
GACAGCATGATTAGTGGTCTTAAACTATGAAGTCATCGCTACAAGCTACACCAATCATAA
CAATTTAAAGTTATGAAATAACTACGAACTTATGTTTCTACGCGTCGTTACTACAAATCT
TCAAATATTTTCGAATTGTAAACATTACTACCTTAAACAAAACAAAAAATCTGTTGTTCC
TTGATCAGATTAATTAATATCTATTGAAATTATTGGT

Last Updated on 06.12.2018 16:11