Sequences from confirmation amplicon


Confirmation sequences for line SALK_074964c and hit At3g13070 are shown.
Line ID SALK_074964c
Locus At3g13070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4194832
Primers used for PCR 60cs - LBb3, show primer details
   
Sequence Name 26-K108893-SALK_074964C-60cs
GenBank Accession LN485025 [GenBank]
Sequencing primer gene specific primer, 60cs: 5'-TTTAGCGAAGTTTCTAGCTCCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K108893-SALK_074964C-60cs
GTTCTTATGATGGGTATGGCACTTGAATTATCTGTTCTGGGTCGATCTGTCATTGACTCT
AAAACCTTAAATTTGAAGAGATATGGTCAAAAATCGAAGCTTTCAGGAAGATTTCTGCCC
AGAGCTGAGCTGCACTGCCCTGTGGCGTTGAGTTCTTCTAAACACTCCAATTTGAGTTTT
AGATTCCGACGAAGTTGTGAATTTAGCTACAGAAGTCGCTTTATGCTGTTTAGCTCCAGT
CAATGTCACGAGGGCAGTCAACGAAAGTCCGATTCTGGTGAACAGGATATATTGTGGTGT
AAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGG
TGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCT
GAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGA
TGGTGGTTCCGAAATCGGCAAAA
   
Sequence Name 10-K108892-SALK_074964C-R210
GenBank Accession LN484543 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K108892-SALK_074964C-R210
GTCATTTGTTACACCACAATATATCCTGTTCACCAGAATCGGACTTTCGTTGACTGCCCT
CGTGACATTGACTGGAGCTAAACAGCATAAAGCGACTTCTGTAGCTAAATTCACAACTTC
GTCGGAATCTAAAACTCAAATTGGAGTGTTTAGAAGAACTCAACGCCACAGGGCAGTGCA
GCTCAGCTCTGGGCAGAAATCTTCCTGAAAGCTTCGATTTTTGACCATATCTCTTCAAAT
TTAAGGTTTTAGAGTCAATGACAGATCGACCCAGAACAGATAATTCAAGTGCCATACCCA
TCATAAGAACCTGATAATAGAAAACCCTAGAGCAAACAAAAACAGAACCTTGGAGCTAGA
AACTTCGCTAA

Last Updated on 06.12.2018 16:11