Sequences from confirmation amplicon


Confirmation sequences for line SALK_075011c and hit At3g01850 are shown.
Line ID SALK_075011c
Locus At3g01850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:300548
Primers used for PCR 27di - LBb3, show primer details
   
Sequence Name 67-K109940-SALK_075011C-27di
GenBank Accession LN485922 [GenBank]
Sequencing primer gene specific primer, 27di: 5'-CTATGTTGACCCCGAAAATAAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K109940-SALK_075011C-27di
AGTACTCAAATACTACTTAACACTGGAATGTTCATGTTGTATACTTGTATTCATAATTAT
AACCATTCCAAAATGACTGAACCTAATACGTTTATTAAATTACTAATTATACATTGCACA
CTACTCTTGGTACCCAACTCCTATGAAATAATCTGTGTGGTGAGGGAATTAATAACTCAA
AATGACGTTATACAGTTCTTGGAACCAAGACTAAGTATCACGATTCACGAGATGATTTTA
AAACATAAAGGACCAGCTTTTTAACCTTGAGAGAAACGAAGAGGTACCATCTTCCGATGA
GAATACAACGCCGTCTCAGCACCACCGCCACCACGTGCAGCTTCTTTTGAAAGGAAAAAA
AAAACAAAAACTATTAACAAAAAGAAGACTTGTAAAAAGCTTTTTTGGATGTATAATATA
CCTATTCTCATCTTACATTTAGAAAATGGGTACTCACTCAAGTGGTGGGTTGGGCTTTCT
CAACACTGGTCCTCAAAAGGGAAATGAAATCTCCTGGCTCCGGAACTCCAAACACTGAAC
TTCCAGCAACAATACAATTGGGGCCTGCTGCAACCCCTAAAA
   
Sequence Name 58-K109939-SALK_075011C-R210
GenBank Accession LN485685 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K109939-SALK_075011C-R210
GTCATTTGTTTTGTCATGTGTTATTTATTCTAAGCGTCGATGTAATTTATTTATTTTTAG
CGGCTGCAGCAGGCGCCAACTGTATTGTTGCTGGAAGTTCAGTGTTTGGAGCTCCGGAGC
CAGGAGATGTCATCTCCCTTCTGAGGACCAGTGTTGAGAAAGCCCAACCCACCACTTGAG
TGAGTACCCATTTTCTAAATGTAAGATGAGAATAGGTATATTATACATCCAAAAAAGCTT
TTTACAAGTCTTCTTTTTGTTAATAGTTTTTGTTTTTTTTTTCCTTTCAAAAGAAGCTGC
ACGTGGTGGCGGTGGTGCTGAAACGGCTTTGTATTCTCATCGGAAGATGGTACCTCTTCG
TTTCTCTCAAGGTTAAAAAGCTGGTCCTTTATGTTTTAAAATCATCTCGTGAATCGTGAT
ACTTAGTCTTGGTTCCAAAAACTGTTTAACGTCATTTTGAGTTATTAATTCCCTCACCCC
ACAAATTATTTCATAGGAATTGGGTACCAAAAGTAGTGGGCAATGGATAATTAGTAATTT
AATAAACGTTTTAGGTTCAGTCATTTTGGAATGGTTATAATTATGAATACCAGTTT

Last Updated on 06.12.2018 16:11