Sequences from confirmation amplicon


Confirmation sequences for line SALK_075550 and hit At5g60360 are shown.
Line ID SALK_075550
Locus At5g60360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24280869
Primers used for PCR 27jd - LBb3, show primer details
   
Sequence Name 24-K113259-SALK_075550C-27jd
GenBank Accession LN484978 [GenBank]
Sequencing primer gene specific primer, 27jd: 5'-AGTTATTGAAAGCTCCAGCACAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K113259-SALK_075550C-27jd
TTCCTTTTCCAATGCCTGATGGTAAGCTGCCTCAAGAGCTCCAGTTGTGCTGCAGTACAA
AAAACAATCATATGACATACAATCTCGACCTTCAATTTGACATCAAAAGATCCCACCAAA
AATTAAAGAGAGTATCTTAATCATTGGTTTTACTTTTACCTGAATGTCCAGCAAGATCCA
CAACCTCCCTGATCTTTGACCGGACTAACGATACCATCTTCTCTCCAGTCTTTCTGCAGT
TACACAATTTGAGAACTCAGAACCAAATTTTTAAAAAGGTCTGCAGCTAAACACGTTCTC
TTAGAGCTGAAAAACAGAACGGGATGGTGGTTGACATAGTTCAGTGCAGCAAGATTGAGT
CCTGGACTTACTGTTTCAGGAAGAGCTGCTTCTGTGACCTTGTGGCTGCCCTTTAAAGTG
GCAGAGCAGTTCTGAGCAGCACCAAGCTTGGTCCTTTGAAACTCTTGCCATGTCAAATCA
AATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTT
TTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAG
AGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTG
GTTCCGAATCGGGCAAAA
   
Sequence Name 07-K113258w-SALK_075550C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K113258w-SALK_075550C-R210
CATTTGATTTGACATGGCAAGAGTTTCAAAGGACCAAGCTTGGTGCTGCTCAGAACTGCT
CTGCCACTTTAAAGGGCAGCCACAAGGTCACAGAAGCAGCTCTTCCTGAAACAGTAAGTC
CAGGACTCAATCTTGCTGCACTGAACTATGTCAACCACCATCCCGTTCTGTTTTTCAGCT
CTAAGAGAACGTGTTTAGCTGCAGACCTTTTTAAAAATTTGGTTCTGAGTTCTCAAATTG
TGTAACTGCAGAAAGACTGGAGAGAAGATGGTATCGTTAGTCCGGTCAAAGATCAGGGAG
GTTGTGGATCTTGCTGGACATTCAGGTAAAAGTAAAACCAATGATTAAGATACTCTCTTT
AATTTTTGGTGGGATCTTTTGATGTCAAATTGAAGGTCGAGATTGTATGTCATATGATTG
TTTTTTGTACTGCAGCACAACTGGAGCTCTTGAGGCAGCTTACCATCAGGCATTTGGAAA
AGGAATTTCTCTCTCTGAGCAACAGCTTGTGGATTGTGCTGGAGCTTCCAATAAC

Last Updated on 06.12.2018 16:11