Sequences from confirmation amplicon


Confirmation sequences for line SALK_076027c and hit At3g50900 are shown.
Line ID SALK_076027c
Locus At3g50900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18918319
Primers used for PCR 21gg - LBb3, show primer details
   
Sequence Name 24-K112064-SALK_076027C-21gg
GenBank Accession LN484974 [GenBank]
Sequencing primer gene specific primer, 21gg: 5'-AAGCTAAGGTGGGAAATGAAGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K112064-SALK_076027C-21gg
CAGCTCTGGGACCGCTTCTAATTAGCAACTACTACGAATTGATTCAGTTCATATAACATA
TCCTTTTAAATAAGAAAACCAAAATCAACTTTTTGATCATATATTAAAGTCAAGACCCTT
GAACTTATCTTATTCTTCACGGAATCTCCATGAGAGTGTACAAAACACTCATCAATTCGT
GCAAAAATGACTAATACATTGTTTGATGCATCTTTTCGAATCGAATCAAGGATTGTTTGG
CTTTCTCATAGATTATATATTTGGAAAAAAAAGACGATATACAAATCTTTTATTCATTTT
CCTTGACGAATCTATCTGTAATGAATCACTGGTGCGTTAGAACTCGGATCCCACACACCT
CCTTCTCTCACATACTCCATATACCTAGCAAACTCTGGTTTCGCCGTTGCTAAACTCTTC
TTCTTCGCCGTATTCTTCTTCTTTCTTTGAAAATGAACGTTGACGCTNAGACAACTTAAT
AACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGG
CAACAGCTGATTGCCCTTCACCGCCNGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT
GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 08-K112049-SALK_076027C-R210
GenBank Accession LN484490 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K112049-SALK_076027C-R210
CGTTATTTTCAAGAAGAAGAAGATACGGCGAAGAAGAAGAGTTTAGCAACGGCGAAACCA
GAGTTTGCTAGGTATATGGAGTATGTGAGAGAAGGAGGTGTGTGGGATCCGAGTTCTAAC
GCACCAGTGATTCATTACAGATAGATTCGTCAAGGAAAATGAATAAAAGATTTGTATATC
GTCTTTTTTTTCCAAATATATAATCTATGAGAAAGCCAAACAATCCTTGATTCGATTCGA
AAAGATGCATCAAACAATGTATTAGTCATTTTTGCACGAATTGATGAGTGTTTTGTACAC
TCTCATGGAGATTCCGTGAAGAATAAGATAAGTTCAAGGGTCTTGACTTTAATATATGAT
CAAAAAGTTGATTTTGGTTTTCTTATTTAAAAGGATATGTTATATGAACTGAATCAATTC
GTAGTAGTTGCTAATATAGAAGCGGTCCCAGAGCTGATATCTTTGAATCATGTTCTATAT
TCTTCATTTCCCACC

Last Updated on 06.12.2018 16:11