Sequences from confirmation amplicon


Confirmation sequences for line SALK_076337c and hit At2g24570 are shown.
Line ID SALK_076337c
Locus At2g24570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10439261
Primers used for PCR 58ew - LBb3, show primer details
   
Sequence Name 12-K111285-SALK_076337C-58ew
GenBank Accession LN484609 [GenBank]
Sequencing primer gene specific primer, 58ew: 5'-ACCGTATCGAACCAAACAATTAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K111285-SALK_076337C-58ew
AATGATTATTTTTGTGGAGTAATAACAATACCTTTTCTTGCATGGCACTTGCCGTTGCCG
GAGCCAGAGATCTTGCCGGAAAAGCCTTCAGAGTGGTCATGTTCAAAGCATCTCTTCCTG
TAAGGAAGACCAGAAAGCGGCGGTTTCCCGGAGGAAGTCACTGGCACCGCTGGAGCCGGA
GCAAGAAAGATCGAAGAGCCTTTAGAGACACTTCCATCACCGGTGATCGCAGAAGACATG
AAAGAAGAGTTAGAAGATACGCTAAAGCTCTCTTTAGCAAACTCAACAACCTCCGAGCTC
TTGGTTTTAGCGCCAAAAACGCTCGGTCTAGTGAAATCTAACGTCACGCTTTGTTGATTT
GCCTGAACGAAGCTAACCGGTGCTGGAGCAGAGATCTGAGTGGGAGCCGGAGTTGTCACT
TTCACCGGTGGAGGAACGGAGGAGGAAGGAGGGGAATGAACCGGACCACGTCTAAACCGG
GCGTGACCGGAACGGTTAAGAAGAGAGATAACTTTCTTGAACTTAGAAACTGTGAAATCA
GTGATCTCAGAGCAATCAACGTTACGTTCTTCGGGACGGTTAGAGAGGACACGAATCAAG
TGTTCCATGCTTTTTAAGCCTTGTGATGCAGCTTCTTGTATAGCCGTTTGATCTTCCATC
TTAGGTAAACGCATAATATCAACGGTCATGATGAGAAACCAGAGGAGAAACTTGAAGATC
TGAGAAGAAATTGACGCTTAGACAACTTAATAACAAATTGACGCTTAGACAACTTAATAA
CACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCA
ACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGG
TTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGA
   
Sequence Name 01-K111284-SALK_076337C-R210
GenBank Accession LN484276 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K111284-SALK_076337C-R210
GTTGTCTAGCGTCAATTTCTTCTCAGATCTTCAAGTTTCTCCTCTGGTTTCTCATCATGA
CCGTTGATATTATGCGTTTACCTAAGATGGAAGATCAAACGGCTATACAAGAAGCTGCAT
CACAAGGCTTAAAAAGCATGGAACACTTGATTCGTGTCCTCTCTAACCGTCCCGAAGAAC
GTAACGTTGATTGCTCTGAGATCACTGATTTCACAGTTTCTAAGTTCAAGAAAGTTATCT
CTCTTCTTAACCGTTCCGGTCACGCCCGGTTTAGACGTGGTCCGGTTCATTCCCCTCCTT
CCTCCTCCGTTCCTCCACCGGTGAAAGTGACAACTCCGGCTCCCACTCAGATCTCTGCTC
CAGCACCGGTTAGCTTCGTTCAGGCAAATCAACAAAGCGTGACGTTAGATTTCACTAGAC
CGAGCGTTTTTGGCGCTAAAACCAAGAGCTCGGAGGTTGTTGAGTTTGCTAAAGAGAGCT
TTAGCGTATCTTCTAACTCTTCTTTCATGTCTTCTGCGATCACCGGTGATGGAAGTGTCT
CTAAAGGCTCTTCGATCTTTCTTGCTCCGGCTCCAGCGGTGCCAGTGACTTCCTCCGGGA
AACCGCCGCTTTCTGGTCTTCCTTACAGGAAGAGATGCTTTGAACATGACCACTCTGAAG
GCTTTTCCGGCAAGATCTCTGGCTCCGGCAACGGCAAGTGCCATTGCAAGAAAAGGTATT
GTTATTACTCCACAATAAATTAATCATTTGTTTGACTAGCCTAGTAGTAGTTAATTGTTT

Last Updated on 06.12.2018 16:11