Sequences from confirmation amplicon


Confirmation sequences for line SALK_077211c and hit At1g10760 are shown.
Line ID SALK_077211c
Locus At1g10760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3586850
Primers used for PCR 54dl - LBb3, show primer details
   
Sequence Name 88-K110041-SALK_077211C-54dl
GenBank Accession LN486316 [GenBank]
Sequencing primer gene specific primer, 54dl: 5'-GGGACAGTAGGAGTAATAGGGACC-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K110041-SALK_077211C-54dl
GAGTTCCACCCTGGGATTTTAGAATTTCTTCATGTAGAGCTTCTCTTCTTATTTCCTTCA
TTGTTCATTCGGAAGTAAAATCTTTTACAGATAATAAGAAATCTAAAGACCAACCTGGAT
GACTAGAATTTCGTCACGGATTCGTTGCCCGACATCTCCTTCACCTCCTCGACCTACAGT
AGACATTATCATCCGCAAAAGTTCTCTGTACTCTGGATAACTTGCATAAACGTCCTGGAG
AAGGTCAGTCAGTCTATCCTGCGCTTTGCTTATCTCCCTGCAGTAAATTGGTGTGAAAAT
TACTGAAACGATGCTGTATATTTATTTAAATATCATTTTCAGATGTCAAAACATATTATA
AGACTCCCATAAGGTTTGCTAATATTTCCCTTGGGGCCGAATAAGAAAGGTGGACAGAGT
CTAAACTTCCAATTAATGTCACATTATTTGAACTGTACCTTGGCTTAACATTATAGTTTT
TGTTCCACACAAGCTGTCTTGTAGCCATAAACCTCATCCAGACTAGGATCCCTGCAAAGC
CCAGTTGACCAGCACTTTTTGCCTCGTCCACAAGATCTGCTGCAATGTTGAATCTAGAAA
AGAAAAAAAATGGCAAGATCCAAAAGAGATGGTGGTGTAAACAAATTGACGCTTAGACAA
CTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGA
GACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTC
CACGCTGGTTT
   
Sequence Name 81-K110040-SALK_077211C-R210
GenBank Accession LN486232 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K110040-SALK_077211C-R210
CGTCACTTTGTTTACACCACCATCTCTTTTGGATCTTGCCATTTTTTTTCTTTTCTAGAT
TCAACATTGCAGCAGATCTTGTGGACGAGGCAAAAAGTGCTGGTCAACTGGGCTTTGCAG
GGATCCTAGTCTGGATGAGGTTTATGGCTACAAGACAGCTTGTGTGGAACAAAAACTATA
ATGTTAAGCCAAGGTACAGTTCAAATAATGTGACATTAATTGGAAGTTTAGACTCTGTCC
ACCTTTCTTATTCGGCCCCAAGGGAAATATTAGCAAACCTTATGGGAGTCTTATAATATG
TTTTGACATCTGAAAATGATATTTAAATAAATATACAGCATCGTTTCAGTAATTTTCACA
CCAATTTACTGCAGGGAGATAAGCAAAGCGCAGGATAGACTGACTGACCTTCTCCAGGAC
GTTTATGCAAGTTATCCAGAGTACAGAGAACTTTTGCGGATGATAATGTCTACTGTAGGT
CGAGGAGGTGAAGGAGATGTCGGGCAACGAATCCGTGACGAAATTCTAGTCATCCAGGTT
GGTCTTTAGATTTCTTATTATCTGTAAAAGATTTTACTTCCGAATGAACAATGAAGGAAA
TAAGAAGAGAAGCTCTACATGAAGAAATTCTAAAATTCCAGGGTTGGAACTCTATTTCTT
GAACTTTCTGGGACTGGGTCCCTATTACTCCA

Last Updated on 06.12.2018 16:11