Sequences from confirmation amplicon


Confirmation sequences for line SALK_078758 and hit At3g19740 are shown.
Line ID SALK_078758
Locus At3g19740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6862977
Primers used for PCR 30gd - LBb3, show primer details
   
Sequence Name 01-K112347-SALK_078758C-R210
GenBank Accession LN484279 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K112347-SALK_078758C-R210
ACCACAAAAGCTGAATTATCATCATTTCTATATCGATGCCTCATATTCTTTGTTACATTG
GATTTTCCGTTATGCGTCATAATCGTTCATTTTAATTGATGTCTGTTCTTAATCAATTAA
TTCCCTGAAGTGTGTTGTTATTCCTCTAGTCGAAATAAATTGTATAGAATCTATGTTTCT
TTCCGAGTTACTATAGTATGTTTTGCGTTGATATTGATCTCGTTTTTTTTTTTTTTAAGG
GGGGAATTTACTTTTGGAAAGCTTTTGGGGGGGAAAAAAAAAAAAAGGGCCCCCGAAAGG
TTTTTCAAAACAAGGGCCCCTTTTTTTTTTTTTGGTTTTTTTTGGAAAAAAAGGGGTTTT
TTTTTTTTTTACGGGGGTTTTAAAAGAAACCCAAAAGGGGGGTTTGTTTTTCCACCACCC
CAATTTTTTTTTGGGGCCCCCTTTTTGGAAAAAAAGGGTTCCAAAATTTGGGGGTTCCCC
AAAAAATTACCCCAAAGGAAAAATTTTAAAAAAAAACCCCCCCTGGGGTTTTTTTCCCCC
CCGGGGGGGTATTTTTCCCCGGGAAAATTTTTTAATTTTTTTAAAAAAACCCCCTTGCGT
TTTTTTTTTTTTCGGGGGGAGGGGAAAAATTTCTTGGGAGGAAAAAAATTTCTTTTAAAA
AAAAAAAAA
   
Sequence Name 10-K112348-SALK_078758C-30gd
GenBank Accession LN484551 [GenBank]
Sequencing primer gene specific primer, 30gd: 5'-TATTACTTTTCTCCTTCCCGGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K112348-SALK_078758C-30gd
TTATCCTCACTAGCATTCCTTCCATCACCCTCAGAACTAAAACTATTCAACTGGCTATTC
TTCAAAATACTTAAATGATTTCCCGAGGCAATAGTACCACCGCGGGGAGAAAATGAACCA
AGTAGAGACTTTCTTGTCAAATTTTCACTATTGGTTAATTTATTTGTGAATCCACAATAT
CTGGAAACCGTATAGTCACGAACGACTGGCCTAACTAAGTACTTGGCTTGCTGCAATACC
AAACCCCATCTCTGGTTCCTCTTTAATGCCCTTGTATACATCACAAAGTCCCTTCTCTTC
CAATATAACTACAAAAAAAAAACCAGAGTTCTTGGTTTTAAAACCCTTTCCGGGGCCCTT
TTTTGGTCCTTCCCACCCAAATGGCTTGGAACCTTGTATTTGCCCCTTTAAAAAAAAAAA
AAAGGATTTTTTTTTCCCCCCAAAAATTTTTTTTTTTCCCCGAAAAAAAAAAAAATTTTT
TAAAATTTTTTTTCCCCCGGGGAAAAAAACCCCCCTTTGGGGGATTTTTTTTTTTAAAAA
CCCCCCTTTTTTAAAAAAAAATTTTTTCCCCCCAAGGGAAAATCCCTTGTTAACCCCAAA
TTGGGGGCCCCCTTTAAAAAAAGAAAAATTTTCCCCTTTTGGGGGGGAAAAAAATTTGCC
CCTAAAAAAATTTAAAAAACCTTGGGGGATTTTTTTAAGGGGGGGGGGGGGTTTTTTTTT
CCCCCGGGAAAGGGGGAAAATTTTTTTTCCCCTTCCCCCCCGGGCCCGAAAAAGTTTCCC
CAAAGGGCCCCCCCTGTTTTTCCCCCAAGGGGAAAAAACCCTTTTTGGGGGGGG

Last Updated on 06.12.2018 16:11