Sequences from confirmation amplicon


Confirmation sequences for line SALK_078797c and hit At5g46590 are shown.
Line ID SALK_078797c
Locus At5g46590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18906305
Primers used for PCR 31dh - LBb3, show primer details
   
Sequence Name 89-K110041-SALK_078797C-31dh
GenBank Accession LN486327 [GenBank]
Sequencing primer gene specific primer, 31dh: 5'-GAAAATCAAAGTCTTGTTGGAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K110041-SALK_078797C-31dh
TTGGATTTGAGGATAGTCCTACAAAATTATAAAACCGGTATACGATTAACAAACATTGGT
TTAGTTAGTTATCAGTATGTTTAGAATTGTGTACACATTACCTTTGAAGAATCGAGGATG
AATTCAGGTGAAATCCAAATGTTGGAAGAATCAGTTTCAGTAGATACAGGATTGGCAAAT
GAGTGAAACGGCATTGTTTCATCGCGACTAGGAGACGTTACTCGGCTTGATAAACCCGAG
CTTTCTCCTGATCCGATTGTTTGCTCGCTTGGAATTTTTCGGATTTTCGTATTAGTTTTG
ATCTCATTCTTCATAGCAACACGACAAATTGACGCTTAGACAACTTAATAACACATTGCG
GACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGAT
TGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCA
GCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCCGGCAAAA
   
Sequence Name 82-K110040-SALK_078797C-R210
GenBank Accession LN486243 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K110040-SALK_078797C-R210
TTGTCGTGTTGCTATGAAGAATGAGATCAAAACTAATACGAAAATCCGAAAAATTCCAAG
CGAGCAAACAATCGGATCAGGAGAAAGCTCGGGTTTATCAAGCCGAGTAACGTCTCCTAG
TCGCGATGAAACAATGCCGTTTCACTCATTTGCCAATCCTGTATCTACTGAAACTGATTC
TTCCAACATTTGGATTTCACCTGAATTCATCCTCGATTCTTCAAAGGTAATGTGTACACA
ATTCTAAACATACTGATAACTAACTAAACCAATGTTTGTTAATCGTATACCGGTTTTATA
ATTTTTGTAGGACTATCCTCAAATCCAAGATGTCGCATCTCAGTGCTTCCAACAAGACTT
TGATTTT

Last Updated on 06.12.2018 16:11