Sequences from confirmation amplicon


Confirmation sequences for line SALK_080513c and hit At2g35710 are shown.
Line ID SALK_080513c
Locus At2g35710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15012262
Primers used for PCR 24ht - LBb3, show primer details
   
Sequence Name 81-K112669-SALK_080513C-24ht
GenBank Accession LN486234 [GenBank]
Sequencing primer gene specific primer, 24ht: 5'-CCATGAGAAACCTAGTGGAAGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K112669-SALK_080513C-24ht
CAGCTCCCGGGAATGTAATCACACTGTTTGGTCCCAGGGTATGTTCCATCTTAGCTTAAG
ATCTGAGAGTACAAGTTAATACATTAGCCAACATTAATCATTATAGACAAAATCTTGAGA
GAAGAATTAAGAGACTACTTACAGAAATAAGAAGCGTCCATTTGGTAGCCTAAGGGAAGT
CTCAAGTGACCATTAAGTACAGATCCGTTACTCGGAGGACTAAAGAGAGGTTGGTCAAGA
AGATCAGAGAAGTAACTGACAAGAAAACCTTGATCAGCTCCATCAGGATTCTTTCTTCCA
ACTTGTAGCTCATGGAGCATGTCCTTGAACACTTCCACTGATGGCTGCAACACGAAGAGA
CCAGTGTGGAAGATACAAGGGTTAATGAAGACCGCACAGAAGCGCCCACACTGGAACAAC
TCGTCGGCTTTCTTAAGAAAGAGGTTATCGGCATCTAGCATGACCACACGGTCGTAATCA
GACAAAGCCCAAGCGTAGAGCTTGTTTAGAGTAAGCTTGAATCTACTGTTGAAGTTGGTC
TGTCTCCTGTATGGATTATCCACATTTTCAACTCTCACCACTTTAGCTCCATCTTCCTCT
TCCCTGCTCACAAAGAGGCTCATTAGTATTCACATCAATAACGTACTTAATAACACATTG
CGGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCA
CCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCGGGCCCTGAGAGAGTTGCAGCA
AGCGGTCCACGCTGGTNTGCCCCAGCAGGCGAANATCCTGTTTGATGGTGGTTCC
   
Sequence Name 71-K112668-SALK_080513C-R210
GenBank Accession LN486031 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K112668-SALK_080513C-R210
TAGTACGTTATTGATGTGAATACTAATGAGCCTCTTTGTGAGCAGGGAAGAGGAAGATGG
AGCTAAAGTGGTGAGAGTTGAAAATGTGGATAATCCATACAGGAGACAGACCAACTTCAA
CAGTAGATTCAAGCTTACTCTAAACAAGCTCTACGCTTGGGCTTTGTCTGATTACGACCG
TGTGGTCATGCTAGATGCCGATAACCTCTTTCTTAAGAAAGCCGACGAGTTGTTCCAGTG
TGGGCGCTTCTGTGCGGTCTTCATTAACCCTTGTATCTTCCACACTGGTCTCTTCGTGTT
GCAGCCATCAGTGGAAGTGTTCAAGGACATGCTCCATGAGCTACAAGTTGGAAGAAAGAA
TCCTGATGGAGCTGATCAAGGTTTTCTTGTCAGTTACTTCTCTGATCTTCTTGACCAACC
TCTCTTTAGTCCTCCGAGTAACGGATCTGTACTTAATGGTCACTTGAGACTTCCCTTAGG
CTACCAAATGGACGCTTCTTATTTCTGTAAGTAGTCTCTTAATTCTTCTCTCAAGATTTT
GTCTATAATGATTAATGTTGGCTAATGTATTAACTTGTACTCTCAGATCTTAAGCTAAGA
TGGAACATACCCTGTGGACCAAACAGTGTGATTACATTCCCGGGAGCTGTTTGGTTAAAG
CCATGGTACTGGTGGTCATGGCCTGTTCTTCCACTAG

Last Updated on 06.12.2018 16:11