Sequences from confirmation amplicon


Confirmation sequences for line SALK_080927c and hit At1g26650 are shown.
Line ID SALK_080927c
Locus At1g26650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9210980
Primers used for PCR 52cy - LBb3, show primer details
   
Sequence Name 19-K109819-SALK_080927C-52cy
GenBank Accession LN484810 [GenBank]
Sequencing primer gene specific primer, 52cy: 5'-CCGACAAAATTGGAAATCTTACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K109819-SALK_080927C-52cy
AATTATTGTTGGACGTAGACAGATCCGTTCCAGATTCGTCTTTTTTTTTCAATTTGATTC
CTGGGTTATCTGAAAGATCTTCTCTTTGCTTCGATTTCTATCTCCGCTGCTTGTGAAATC
AACCTTTATGGTATGTAATCTCGAACTCAGCTAAAACACACTTCACTCTCGGAAACATGG
AGACGGAAACGAATCAGGTTTTACGGAAACCGGAGCTAAAATCATACAGTCACAAACAAT
TTCACTCCTCAAATGCGTTAGAGATCTTAAGAGAAACAGTTCGAATTCTCCGGTACAATC
TTGGTGCTTTAATGTTAACTACTGCTGTTTTGATCTGTCCTGTCTCTGCTTTGCTCTTAC
CAAACTTTTTAGTTGATCAATCATTAGTGAATAAACTCACAGTGAAGCTTCTTTTAGTGG
CGAAATCAAGTGGTCTTCCTTTGCAGCCTTTTGTCAAACATTCTTGTCAGAAATTCGCTG
AAACTGCTGTTTCGTCTGCAATGTGTTTCCCTGTGTTTATCACTGTGTCTCTGTTGTCTA
AAGCAGCTGTGGTTTACTCAGTGGATTGTTCTTACTCGAGGGA
   
Sequence Name 03-K109818-SALK_080927C-R210
GenBank Accession LN484332 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K109818-SALK_080927C-R210
ATTTGTTACCCGCAATGTGCAGATTATAATCGCGTTAGCGAAAACTACAGAGAAGGCTAA
ACCAACTAGCATAGCTCCATAAACATTGAAATCTGGAGAGAAGCCGAGAACAGAGAAGGA
ACTGCAGATTGCTACAAGAAGAACGCAAAAGAAAGTGAAGCAACCAACAATCAGAATGCA
AATCCAAACGTAAGTGAACACAACTCTCCTCCATATCTTCTGCAATATAACCAAGAACTT
GCTTATATCAACTACTTCCCTCGAGTAAGAACAATCCACTGAGTAAACCACAGCTGCTTT
AGACAACAGAGACACAGTGATAAACACAGGGAAACACATTGCAGACGAAACAGCAGTTTC
AGCGAATTTCTGACAAGAATGTTTGACAAAAGGCTGCAAAGGAAGACCACTTGATTTCGC
CACTAAAAGAAGCTTCACTGTGAGTTTATTCACTAATGATTGATCAACTAAAAAGTTTGG
TAAGAGCAAAGCAGAGACAGGACAGATCAAAACAGCAGTAGTTAACATTAAAGCACCAAG
ATTGTACCGGAGAATTCGAACTGTTTCTCTTAAGATCTCTAACGCATTTGAGGAGTGAAA
TTGTTTGTGACTGTATGATTTTAGCTCCGGTTTCCGTAAAACCTGATTCGTTTCCGTCTC
CATGTTTCCGAGAGTGAAGTGTGTTTTAGCTGAGTTCGAGATTACATACCATAAAGGTTG
ATTTCACAAGCAGCGGAGATAGAAATCGAAGCAAAGAGAAGATCTTTCAG

Last Updated on 06.12.2018 16:11