Sequences from confirmation amplicon


Confirmation sequences for line SALK_081332 and hit At4g29550 are shown.
Line ID SALK_081332
Locus At4g29550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14502591
Primers used for PCR BI88 - R204, show primer details
   
Sequence Name 09-K107313-SALK_081332C-BI88
GenBank Accession LN484511 [GenBank]
Sequencing primer gene specific primer, BI88: 5'-ATCCTCAGGTCTTCATCTTTTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K107313-SALK_081332C-BI88
CTGGAAGTGAACGTGAATCGTTGGCTGGATCACAGCCTCAGCAGTAATGTTGCAAGTCAT
AAGCGCACAGAATCTAGAATTATATTTATCGACGTGCATCAACTCAAGGTTTGAGCCCTG
AAATCAGAGGTAGTAAAGAAGATAAAGAAGAATTCAGTAAAAAAATACAAGTAGTGTGGT
TAGACCAAGATGAGAATTTTTAACCTTTTGAAAGTTGTACCAATGGAGTCCCACTCTAGC
ACAAATGCCAACAATGTCACCACGAAGGTCATCATTGTTGGCACATTTGTACACATAAAG
TGAACTAGTCGGAATACGTATGTCAACATCAAATCCCTGCATGCGAGAATTATTATCAAA
AGTCACACATGAACAAGTTGAGATCAGGGAAGATCCTGTATATATATGTATATCTTTACG
TCGGATTCGCGAATTTGGCGATAGAACTCTTTCTCCAAAGCCGGGTCAACCTGATCATCA
TCATCTTCGTCAGTCCATCCAGATGGTGGATCGTTATGTACAGCGTCAGACAATTCTTCT
TTCGATATAAAACAAATTGTGGTGGATGTTTATTTTGGTGTAAACAAATTGACGCTTAGA
CAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAG
TGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAG
   
Sequence Name 49-K107289-SALK_081332C-R210
GenBank Accession LN485472 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K107289-SALK_081332C-R210
GTGCTAGCGTCATTTGTTTCACCAAAATAACATCCACCACAATTTGTTTTATATCGAAAG
AAGAATTGTCTGACGCTGTACATAACGATCCACCATCTGGATGGACTGACGAAGATGATG
ATGATCAGGTTGACCCGGCTTTGGAGAAAGAGTTCTATCGCCAAATTCGCGAATCCGACG
TAAAGATATACATATATATACAGGATCTTCCCTGATCTCAACTTGTTCATGTGTGACTTT
TGATAATAATTCTCGCATGCAGGGATTTGATGTTGACATACGTATTCCGACTAGTTCACT
TTATGTGTACAAATGTGCCAACAATGATGACCTTCGTGGTGACATTGTTGGCATTTGTGC
TAGAGTGGGACTCCATTGGTACAACTTTCAAAAGGTTAAAAATTCTCATCTTGGTCTAAC
CACACTACTTGTATTTTTTTACTGAATTCTTCTTTATCTTCTTTACTACCTCTGATTTCA
GGGCTCAAACCTTGAGTTGATGCACGTCGATAAATATAATTCTAGATTCTGTGCGCTTAT
GACTTACAACATTACTGCTGAGGCTGTTGATCCAGCCAACGATTCACGTTTCACTTTCCA
GACATGTGTTACCAGAGCTACATGTCAAAAAGATGAAGACCTGAGGA
Primers used for PCR 61xs - R212, show primer details
   
Sequence Name 87-K119375-SALK_081332C-61xs
GenBank Accession LN486312 [GenBank]
Sequencing primer gene specific primer, 61xs: 5'-GCTCGAGTTACGGGTAACAGTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K119375-SALK_081332C-61xs
CTCAGTTTTAACATGTGCATTCTATAGTCTTTGGCATCTACTTACTTTAGGTTTCATGAT
TGACGACATTTTTGCTTGTTTCTCCTGCGGATTAAGAAAAGTTAGTTAGAAAACCAAGAA
TCTTGTTTTTTCTATGCATAGCTATCGATCCTTTTACCAGATACACCAAAAAAAAGAAAC
AAGATCAACACTACATAAGCCAAGTATTAGCATCCATTTAAACAAATCCAGGCTCAATTC
TACTAGACCCGATGAATAACCCAAAGATCTCTGCTCAGAACAGAGCATACCAAAGAAAAA
AAGAAAGAAAGAGAGGAGATGCAATGATTTCATTACGCTAGCTAGACCTAACTGTTACAT
ACGTTCGCTATAGTTTCTGGCTTTCTGTACACGGAATGCAAAGAAAGGGGGAAAACAAAA
ATTTGAGAAAAGATAAAAGACGTTACTGTTGACCGGTGACCAAGTAGACAATAGCCGGCG
ACGAAATAAGCGGGAATATCGTTATCCAATTTGACGATTGGTCCAAAACCAAATGAGCCT
AGCAGAGCGGAATGAAAATACATCGGTCTAAACGACGGCGTTTATCTGAATATTTATTGC
CTTTCTACTTGTCTCCGTTGCCATCCGCCAAATCGACGAAGACGACGATTACCAGGTTCA
TGAAATCTGCTTCTCACTTTTATTTTAGCCATCGTAGATCTATTTTCTTATGGTCAATTC
GCTTCAGTGTTTTGAAATTTTGAAGCCACAGCTTTCTGGATTTGTAATTTCGAATATGAA
AATCGACTGAAATTGTTTTAGGATATGGAAGCAGTCGATCGACCGCTGCGCAT
   
Sequence Name 83-K119374-SALK_081332C-R214
GenBank Accession LN486265 [GenBank]
Sequencing primer gene specific primer, R214: 5'-AACGTTGCGGTTCTGTCAGTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K119374-SALK_081332C-R214
GGGGTCATACGTGACTCCCTTAATTCTCCGCTCATGATCAGATTGTCGTTTCCCGCCTTC
AGTTTAAACTATCAGTTTATTTTGGTGTAAACAAATTGACGCTTAGACAACTAAATACAT
CGTCATTATCATCATCTTCGTCAGTCCATCCAGATCCAGATGGTGGATGTTTATTTAACC
ATTCTTCTTTCGATATAAAACCGCAACTAGATACATCATGAGATACGGTGGTTTCTTCAA
TGCGCAGCGGTCGATCGACTGCTTCCATATCCTAAAACAATTTCAGTCGATTTTCATATT
CGAAATTACAAATCCAGAAAGCTGTGGCTTCAAAATTTCAAAACACTGAAGCGAATTGAC
CATAAGAAAATAGATCTACGATGGCTAAAATAAAAGTGAGAAGCAGATTTCATGAACCTG
GTAATCGTCGTCTTCGTCGATTTGGCGGATGGCAACGGAGACAAGTAGAAAGGCAATAAA
TATTCAGATAAACGCCGTCGTTTAGACCGATGTATTTTCATTCCGCTCTGCTAGGCTCAT
TTGGTTTTGGACCAATCGTCAAATTGGATAACGATATTCCCGCTTATTTCGTCGCCGGCT
ATTGTCTACTTGGTCACCGGTCAACAGTAACGTCTTTTATCTTTTCTCAAATTTTTGTTT
TCCCCCTTTCTTTGCATTCCGTGTACAGAAAGCCAGAAACTATAGCGAACGTATGTAACA
GTTAGGTCTAGCTAGCGTAATGAAATCATTGCATCTCCTCTCTTTCTTTCTTTTTTTCTT
TGGTATGCTCTGTTCTGAGCAGAGATCTTTGGGTTATTCATCGGGTCTAGTAGAATTGAG
CCTGGATTTGTTTAATGGATGCTAATACTTGGCTTATGTAGTGTTGATCTTGTTTCTTTT
TTTGGTGTATCGG

Last Updated on 06.12.2018 16:11