Sequences from confirmation amplicon


Confirmation sequences for line SALK_081563c and hit At4g29840 are shown.
Line ID SALK_081563c
Locus At4g29840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14601052
Primers used for PCR 11ds - LBb3, show primer details
   
Sequence Name 25-K112623-SALK_081563C-11ds
GenBank Accession LN485004 [GenBank]
Sequencing primer gene specific primer, 11ds: 5'-GCCCTTCTAACCTCAAACTATTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K112623-SALK_081563C-11ds
AGTTTCTCTCTATCAGCTTCATACACCCATCAAAATCAGTGTCAATACTCAAAACAAACG
CACCATTAGCTATCGGCTGAACCAGCTGAGCCATAGAGATCTTGTTCGCCGGTAAAAACA
CAATCGATGGGATTCCAGCGGAGGCGCAGTAAGCAGATAGAGCAGCAGAAGTATCTCCGG
TGGAAGCACATCCGACACCAACCACAGGTCGTTTCATCTTTCTCAGACGATTAACTTGAC
TAACCAAAACAGTCATTCCAAGATCCTTGAAACTTCCTGTATGACTAATCCCACAGTGTT
TCACCCACAGATCATTCATACCTAGAAACTGCTTACCAAATCTCTCTGCCCAGAACAGAT
TCGAGTTTCCTTCAAAAGCTGAAACGATGTCGTCGTCATCGATCTCAGGAAGAACCCACT
CTTTCTTCGACCAAACACCCGATCCATAAGGCCATGTGCTTTTACCAACACGCGAATCGA
AGAGATCACGCCAATACGCGCCATCGAATCGCTTCAAAGCCTCCATATCGTGTTCGACAT
CAAGCAAACCACCGGAGCGGCTACGGTACACGATCTCGTCGAGAGAGTAAGACTCCGTGG
ATCCAGGAGCTGCATTAAACGGAACGTATTTAGCTGAAAATGGATTCACGGCGTTGGAAC
GATTACGACGAGCCTCATCTCGAATGTTATCCTCGGTACGGTGAGGAGGCTTGACCGCTG
TCTCGATCGGGGCTTTGATGTTGTTGCCATCGGCGGTACAGGAGATGACGACGGGGCGGT
GGCGGAGGAGAGAGGTTGACCGGTGGCGGCGGATGGGGTCTTGTTTAGGGTTTAAGGACG
ATACAGAGGCATTGAAGAGACAAGACGAAGCCATTTTTTCTAGAGAATGAGAAGTGAGAT
GCGGCTGAGGATATATTGTGGTGTAAACAATTGACG
   
Sequence Name 01-K112622-SALK_081563C-R210
GenBank Accession LN484280 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K112622-SALK_081563C-R210
CATTTGTTTCCCACAATATATCCTCAGCCGCATCTCACTTCTCATTCTCTAGAAAAAATG
GCTTCGTCTTGTCTCTTCAATGCCTCTGTATCGTCCTTAAACCCTAAACAAGACCCCATC
CGCCGCCACCGGTCAACCTCTCTCCTCCGCCACCGCCCCGTCGTCATCTCCTGTACCGCC
GATGGCAACAACATCAAAGCCCCGATCGAGACAGCGGTCAAGCCTCCTCACCGTACCGAG
GATAACATTCGAGATGAGGCTCGTCGTAATCGTTCCAACGCCGTGAATCCATTTTCAGCT
AAATACGTTCCGTTTAATGCAGCTCCTGGATCCACGGAGTCTTACTCTCTCGACGAGATC
GTGTACCGTAGCCGCTCCGGTGGTTTGCTTGATGTCGAACACGATATGGAGGCTTTGAAG
CGATTCGATGGCGCGTATTGGCGTGATCTCTTCGATTCGCGTGTTGGTAAAAGCACATGG
CCTTATGGATCGGGTGTTTGGTCGAAGAAAGAGTGGGTTCTTCCTGAGATCGATGACGAC
GACATCGTTTCAGCTTTTGAAGGAAACTCGAATCTGTTCTGGGCAGAGAGATTTGGTAAG
CAGTTTCTAGGTATGAATGATCTGTGGGTGAAACACTGTGGGATTAGTCATACAGGAAGT
TTCAAGGATCTTGGAATGACTGTTTTGGTTAGTCAAGTTAATCGTCTGAGAAAGATGAAA
CGACCTGTGGTTGGTGTCGGATGTGCTTCCACCGGAGATACTTCTGCTGCTCTATCTGCT
TACTGCGCCTCCGCTGGAATCCCATCGATTGTGTTTTTACCGGCGAACAAGATCTCTATG
GCTCAGCTGGTTCAGCCGATAGCTA

Last Updated on 06.12.2018 16:11