Sequences from confirmation amplicon


Confirmation sequences for line SALK_081725c and hit At5g45180 are shown.
Line ID SALK_081725c
Locus At5g45180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18275244
Primers used for PCR 14ke - LBb3, show primer details
   
Sequence Name 77-K113914-SALK_081725C-14ke
GenBank Accession LN486174 [GenBank]
Sequencing primer gene specific primer, 14ke: 5'-TATCAAATTAGCACAACATTTCGG-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K113914-SALK_081725C-14ke
AAAAAGTGTATTAAAAAAGTGTACGTACCTGTATAAAGGCATGATACCCCAAGGAAACTC
AAGCCATGATCGAAAAGGTTCAGGGACAATGGCTTTTGAGCTTCTTCATGCCATCATATC
CTGTCGCAAGTATCACAACATCAGCTTCTAGGGTAGTGCCATCCTCAAACTCAATCCCTT
CATCATAAAACCACCAATTTGTTGTCCTTTTGAAACGGATCATTCCCTTATCCGCTTCTT
CAAAAAATTTCTCTGGCACGAGCGCCATTTGACAAGAAGCATAGTCCTCCTCGAATGCAT
GGTCCGGTTTAAAACCATATTTCTCTAACGGAAGCTTCCACAAAACATATGATTCGATGA
ATTTGGAAACTGTGGCACGCTATGTTTGTCAAATTGACGCTAAAACAACTTAATAACACA
TTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAG
CTGATTGCCCTTCACCGCCGGGCCCTGAAAAAGTTGCAGCAAGCGGTCCACGCTGGTTTG
CCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCC
   
Sequence Name 66-K113913-SALK_081725C-R210
GenBank Accession LN485902 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K113913-SALK_081725C-R210
CGTCATTTGACAACATAGCGTGCCACAGTTTCCAAATTCATCGAATCATATGTTTTGTGG
AAGCTTCCGTTAGAGAAATATGGTCTTAAACCGGACCATGCATTCGAGGAGGACTATGCT
TCTTGTCAAATGGCGCTCGTGCCAGAGAAATTCTTTGAAGAAGCGGATAAGGGAATGATC
CGTTTCAAAAGGACAACAAATTGGTGGTTTTATGATGAAGGGATTGAGTTTGAGGATGGC
ACTACCCTAGAAGCTGATGTTGTGATACTTGCGACAGGATATGATGGCATGAAGAAGCTC
AAAGCCATTGTCCCTGAACCTTTTCGATCATGGCTTGAGTTTCCTTGGGGTATCATGCCT
TTATACAGGTACGTACACTTTTTTAATACACTTTTTTTTTTATATAGTAAACTTGTTCTA
AACTATTCCAAAATGT

Last Updated on 06.12.2018 16:11