Sequences from confirmation amplicon


Confirmation sequences for line SALK_082525c and hit At1g74750 are shown.
Line ID SALK_082525c
Locus At1g74750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28087942
Primers used for PCR 38cv - LBb3, show primer details
   
Sequence Name 60-K109345-SALK_082525C-38cv
GenBank Accession LN485736 [GenBank]
Sequencing primer gene specific primer, 38cv: 5'-GAGTACAACCTTGACCAACCATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K109345-SALK_082525C-38cv
TATGGCCCGCTTTTCAGACAGTTTATTATAACACTGTAGGTGAAGGTATCAGGAGAAAGC
CCTGCTTCTTGCATTCTTTGGTACATATCCATGGCAATGTCAAGAAATCCAGCTTTAGCA
TGGATATCTATCAGGGTACAGTAAGTTACACGGTCAGGCTCACAACCAGCCTCTTGCATC
TGATTAAAAACATTCATCGCTTCTTTGAGGTAATTTGCACGCCCGTAGCTATGGATGAGT
CGGTTATATGTAACAGTATTTGGCTTACACCCATCCCTAACCATCTCATCAAGATATATT
GTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGT
ACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCC
TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCC
TGTTTGATGGTGGTTCCGAATCGNGCAAAA
   
Sequence Name 52-K109344-SALK_082525C-R210
GenBank Accession LN485554 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K109344-SALK_082525C-R210
GTCATTTGTTTACCCCAATATATCTTGATGAGATGGTTAGGGATGGGTGTAAGCCAAATA
CTGTTACATATAACCGACTCATCCATAGCTACGGGCGTGCAAATTACCTCAAAGAAGCGA
TGAATGTTTTTAATCAGATGCAAGAGGCTGGTTGTGAGCCTGACCGTGTAACTTACTGTA
CCCTGATAGATATCCATGCTAAAGCTGGATTTCTTGACATTGCCATGGATATGTACCAAA
GAATGCAAGAAGCAGGGCTTTCTCCTGATACCTTCACCTACAGTGTTATAATAAACTGTC
TTGGAAAAGCGGGCCATTTACCTGCTGCCCATAGGCTCTTTTGTGAAATGGTTGGTCAAG
GTTGTACT

Last Updated on 06.12.2018 16:11