Sequences from confirmation amplicon


Confirmation sequences for line SALK_083120 and hit At2g36310 are shown.
Line ID SALK_083120
Locus At2g36310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15226183
Primers used for PCR AW33 - R204, show primer details
   
Sequence Name 73-K106742-SALK_083120C-R210
GenBank Accession LN486071 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K106742-SALK_083120C-R210
TGCATCAGTTACTCTGTCACTACAACTTTTGGAATGCGTCCGTGATCTCTTTCTCTTTGT
TCATTTTGCAAAAGAATAGTGTGAGATTGCTGGCTTCCCTGATGTTCCCGTTGCAGAAGG
AAGTTCCGAACCTTTAAAGGTACCATCTTTCTCTCCTCTCATAAGGATTGAAACTTTAGT
TTATGCCATGAAGATTTTTCAGATCTTTGCTATGAGACAAAAAGCCAAACCGTTGTGACT
TTAGCTTGAATTCTGGCTTTGGTGTGTTGATTCAGGGTGGAATTCCGCGTGTTGCTGATT
TTGTGCATGGTAAAAACGGACTAGGAGATGTCTCTCTTCCTCCTCCGAGTAGAAAGAAAT
CTGAGAAAAGTGCAGCTGAGTTTCTAGATGAGAAGGTCGAAGAATATCCGGGTGAAGTCA
CCATTCTCGCCCTCGGACCTCTAACCAACCTGGCATTAGTAAGAATCATGAATCCCCTCC
TTTAATCTTCTTAAAATCCGATTTGTGTACTGACGATGACCGGGTTTTTTCGTTATAGGC
CATCAAACGCGATAGTTCATTTGCGAGCAAGGTGAAGAAAATTGTTATTCTTGGTGGAGC
TTTCT
   
Sequence Name 89-K106743-SALK_083120C-AW33
GenBank Accession LN486323 [GenBank]
Sequencing primer gene specific primer, AW33: 5'-AGAAAGCTCCACCAAGAATAACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K106743-SALK_083120C-AW33
CGCGTTTGATGGCCTATAACAAAAAAACCCGGTCATCGTCAGTACACAAATCGGATTTTA
AGAAGATTAAAGGAGGGGATTCATGATTCTTACTAATGCCAGGTTGGTTAGAGGTCCGAG
GGCGAGAATGGTGACTTCACCCGGATATTCTTCGACCTTCTCATCTAGAAACTCAGCTGC
ACTTTTCTCAGATTTCTTTCTACTCGGAGGAGGAAGAGAGACATCTCCTAGTCCGTTTTT
ACCATGCACAAAATCAGCAACACGCGGAATTCCACCCTGAATCAACACACCAAAGCCAGA
ATTCAAGCTAAAGTCACAACGGTTTGGCTTTTTGTCTCATAGCAAAGATCTGAAAAATCT
TCATGGCATAAACTAAAGTTTCAATCCTTATGAGAGGAGAGAAAGATGGTACCTTTAAAG
GTTCGGAACTTCCTTCTGCAACGGGAACATCAGGGAAGCCAGCAATCTCACACTATTCTT
TTGCAAAATGAACAAAGAGAAAGAGATCACAGACGCATTCCAAAAGTTGTAGTGACAGAG
TAAACTGATGCATTGTAATATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTT
TAATGTACTGGGGTGGTTTTTCTTTTCACCANTGAGACGGGCAACAGCTGATTGCC
Primers used for PCR 78xo - LBb3, show primer details
   
Sequence Name 66-K119296-SALK_083120C-78xo
GenBank Accession LN485915 [GenBank]
Sequencing primer gene specific primer, 78xo: 5'-AATAAGAAAATTGTGGCTGGACG-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K119296-SALK_083120C-78xo
CTAATAACTTCGATTCTGCTTTTTATATAAAGTTGAGGTTTTTGAGCTATTTTTTCTCCT
AAAGGATTCCGAAAAAAGCTTAGACCTTTTTGATCTGATACGTATCTATAAATCTGGAAA
GGAGCTGAGAGTTTCAAGGGGTTTTAATTTCGCTGAGAAAACGTTTAATTATCTTTGTCG
AGATGGATTGTGGTATGGAGAATTGTAATGGTGGGATCTCAAACGGTGACGTTTTGGGTA
AGCATGAGAAGCTCATTATCGATACAGACCCAGGAATTGGTGAGCTTCTTCTTCCCCTGT
TTTGATTACGTCTTTTAGTAAATGGATCTTCAGGCAGAGTAGGATTTAACCTCAGAAAGC
TTCCCACTTTTGTTTAATTTGAATAGAAATAT
   
Sequence Name 18-K119295-SALK_083120C-R210
GenBank Accession LN484801 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K119295-SALK_083120C-R210
ACATTACCAAACACAGTAGTTAATCCTAATATCTCCAGCTCCGGTGTTTGAAACGCCATC
AATATCGCCATGCTATCATCTAATCATACACTAATGTTAGAACTGATCCTATTGAACAGT
CATTTGGAAAAAAGTCAACACACAAAAAAGAACAATTTCCCCTGAATCTTTCTATTAAGC
AAAGCATTGAACTTTACAAGTAAATGAGCACACAAAAACAGATTCAAGAGATAACCATCT
AAATATTTCTATTCAAATTAAACAAAGTTGGAAGCTTTCTGAGGTTAAATCCTACTCTGC
CTGAAGATCCATTTACTAAAAGACGTAATCAAAACAGGGGAAGAAGAAGCTCACCAATTC
CTGGGTCTGTATCGATAATGAGCTTCTCATGCTTACCCAAAACGTCACCGTTTGAGATCC
CACCATTACAATTCTCCATACCACAATCCATCTCGACAAAGATAATTAAACGTTTTCTCA
GCGAAATTAAAACCCCTTGAAACTCTCAGCTCCTTTCCAGATTTATAGATACGTATCAGA
TCAAAAAGGTCTAAGCTTTTTTCGGAATCCTTTAGGAGAAAAAATAGCTCAAAAACCTCA
ACTTTATATAAAAAGCAGAATCGAAGTGGATTAGTTATGAAATAATAAACAAGATATATG
GAAAGAAGACGACGAATTGCTGAGTCGTCCAGCCACATTTTTCTTA

Last Updated on 06.12.2018 16:11