Sequences from confirmation amplicon


Confirmation sequences for line SALK_083158c and hit At4g33370 are shown.
Line ID SALK_083158c
Locus At4g33370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16069687
Primers used for PCR 64us - LBb3, show primer details
   
Sequence Name 28-K117668-SALK_083158C-R210
GenBank Accession LN485094 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K117668-SALK_083158C-R210
CATTTGTTACACCACAATATACAGAGGAGAGGTGTAATCTTGTTTCAGGTTCGTGCTCTA
ATCTTGTTTCGAACTAGCTATCACCGAATTGAGATTTCTTTACTCATTATGTCATCTTGT
ATTTTCGTGGATCTGTCTGTAATTTGAAATTTTGTTTCACTCAAATGAGAAACTTACTAG
TTCTTTGTTATCTGTAGTATGTGACAAAACAAAACACAAAAGAATCTTGCTATTGGTTGA
TCCAGCTTTCAGACAATGATGTTTGTATTAAATATTAATTAATTAAACCCTCTCAACCAA
TTAGAGGTTTCTTGGCTTAGAAAAGCAACGATGGGCCCAAAGATGCCCAACACAGAAACA
GAAAATATGAAAATATTAGTCACCGGCTCTCNTGGTTACT
   
Sequence Name 65-K117669-SALK_083158C-64us
GenBank Accession LN485882 [GenBank]
Sequencing primer gene specific primer, 64us: 5'-AGTAACCAGTAGAGCCGGTGACTA-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K117669-SALK_083158C-64us
GCACTTTGGGCCATCGTTGCTTTTCTAGCCAAGAAACCTCTAATTGGTTGAGAGGGTTTA
ATTAATTAATATTTAATACAAACATCATTGTCTGAAAGCTGGATCAACCAATAGCAAGAT
TCTTTTGTGTTTTGTTTTGTCACATACTACAGATAACAAAGAACTAGTAAGTTTCTCATT
TGAGTGAAACAAAATTTCAAATTACAGACAGATCCACGAAAATACAAGATGACATAATGA
GTAAAGAAATCTCAATTCGGTGATAGCTAGTTCGAAACAAGATTAGAGCACGAACCTTGA
AACAAGATTACACCTCTCCTCTGTATATTGTGGTGTAAACAAATTGACGCTTAGACAACT
TAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGA
CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCA
CGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCC

Last Updated on 06.12.2018 16:11