Sequences from confirmation amplicon


Confirmation sequences for line SALK_083569c and hit At1g34120 are shown.
Line ID SALK_083569c
Locus At1g34120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12427394
Primers used for PCR 12gq - LBb3, show primer details
   
Sequence Name 73-K112564-SALK_083569C-12gq
GenBank Accession LN486076 [GenBank]
Sequencing primer gene specific primer, 12gq: 5'-TTGGGGATTGATACAAATGGTAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K112564-SALK_083569C-12gq
TATTATAAGGATTAAAATTTTATATGATATTTTTGAAAGTTTCAAAAATTATCATTTTTA
AAATAATAAAGTTTTTTAGTGTCATTTGTGTCAATCCCCCCGCATTTAATCTATTAATAA
TCTGACTTATTTTCTCCCCAATTTAGTTGACATATTCTTAACCAATTTTAAACCGGAGTA
TAATCAAGCTTTTAACCGCCGGTTCGAAAAAACAATCAATATTTTCAATCTGTCTCCTAC
CAATGGTCGTTAAGTCTTTTCTTCTTCCTCCTGCAACCTCTTCTTCCCTCTCTCAGTTTG
ATTTGACCGGAGTATATGGCGGAAGTACGATCACGATCACGGAGAACGGAGGTATCCAGA
TCTATATCTTCTCCTTTGCTTCTTCTGCTCGTATAGTTTGGTTAAATTAGGTTTTCCGAA
TTTTGGGGATAACGATCATGTGAAATGTTGCAGAGCAATTGGGCTACAATATGCTGCTCA
GCTTTCTCGTGTCTCCAGCTCTACTGGGCACGTATTGTTCTCCGTAAATGGTTCAATGTT
TCTGCTTCTGAATCAGATTATAGTGCTGATAGTGACGATGACTACGAAGATAGATCACAA
GGTCAGCTTAAACTCTGATTCTCCGGAAATAATTAGGTTTTGTATTCTACTGTGACTGAA
TCTGAATTTGGTGTAGCAGAATTTGATCCAATTAGCTCTGGAGTTACCAATCCTCGTGTT
GATACTGACGGTAATGTGATATGTAACTCTCAGATTTCAATTTTTATTTCTAGAATCATT
CATTGTTGCTAATTTTCGGTGATGATGTTTTCATTAGAAGAACATTATTGCTCATTCAGA
TCCATTTTTCTTAATTTCAGATAGGCCTAAGCTTAGAAGCACTGATAGTTTAAACTGAAG
GCGGGAAACGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTT
TAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGGCACAGCTGAATGCCCTTC
ACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGGTCACGCTGGTTTGCCCCAGCAGCGAA
AA
   
Sequence Name 57-K112563-SALK_083569C-R210
GenBank Accession LN485663 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K112563-SALK_083569C-R210
CATTTGTTTACCGTTTCCCGCCTTCAGTTTAAACTATCAGTGCTTCTAAGCTTAGGCCTA
TCTGAAATTAAGAAAAATGGATCTGAATGAGCAATAATGTTCTTCTAATGAAAACATCAT
CACCGAAAATTAGCAACAATGAATGATTCTAGAAATAAAAATTGAAATCTGAGAGTTACA
TATCACATTACCGTCAGTATCAACACGAGGATTGGTAACTCCAGAGCTAATTGGATCAAA
TTCTGCTACACCAAATTCAGATTCAGTCACAGTAGAATACAAAACCTAATTATTTCCGGA
GAATCAGAGTTTAAGCTGACCTTGTGATCTATCTTCGTAGTCATCGTCACTATCAGCACT
ATAATCTGATTCAGAAGCAGAAACATTGAACCATTTACGGAGAACAATACGTGCCCAGTA
GAGCTGGAGACACGAGAAAGCTGAGCAGCATATTGTAGCCCAATTGCTCTGCAACATTTC
ACATGATCGTTATCCCCAAAATTCGGAAAACCTAATTTAACCAAACTATACGAGCAGAAG
AAGCAAAGGAGAAGATATAGATCTGGATACCTCCGTTCTCCGTGATCGTGATCGTACTTC
CGCCATATACTCCGGTCAAATCAAACTGAGAGAGGGAAGAAGAGGTTGCAGGAGGAAGAA
GAAAAGACTTAACGACCATTGGTAGGAGACAGATTGAAAATATTGATTGTTTTTTCGAAC
CGGCGGTTAAAAGCTTGATTATACTCCGGTTTAAAATTGGTTAAGAATATGTCAACTAAA
TTGGGGAGAAAATAAGTCAGATTATTAATAGATTAAATGCGGGGGGATTGACACAAATGA
CACTAAAAAACTTTATTATTTTAAAAATGATAATTTTTGAAACTTTCAAAAATATCATAT
AAAATTTTTAATCCTTTATAAATTAATTAAATAATAAAGTTTTTTTATTACCATTTGT

Last Updated on 06.12.2018 16:11