Sequences from confirmation amplicon


Confirmation sequences for line SALK_083793c and hit At2g38700 are shown.
Line ID SALK_083793c
Locus At2g38700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16181128
Primers used for PCR 43fx - LBb3, show primer details
   
Sequence Name 25-K112064-SALK_083793C-43fx
GenBank Accession LN485002 [GenBank]
Sequencing primer gene specific primer, 43fx: 5'-ATTTATCGTTGTGACACTGCTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K112064-SALK_083793C-43fx
GCTACGCATAGATCTGGCTCCATCACCAGTTTACTTGTTTTTATCGCCGTGGGAAGTCAT
ATGATTACTATAACAAAGGATTACTACAGCAGCCTACAACACAACCAATAAAAAAAAAAC
CACACTTTCTCCCTTGCACAAAGTTAACTTATTCATAATTCGTGTCACACAAGACCATAA
TAACCATATTTTGAAAAGTTGCAAACTTGGACTAAAAACAAACAGTTGATGGAGGAATCA
AATTAAATACTACATGGTGCATAGACAATAATCAAATTCAGTGAATAGAGATTAAACTAG
GGAAATAAGAAACAGGAATTCCGATGGAGTCACAAGTGAAAGCAGCAACACATGATTTGG
TTTCGAGATCTTGTATGGTTAGTGTTTTTATACGATATGGACTACAGTACAACAACTTCT
TCTTGTTCTGGATTTGAATGATACACTCAAATTTAGCATCAAGATCTTATTCGTTTGCAA
TATTGATTGTTTTGTTTTTTGCACCCAATGCAAAAAAATTGTACGTAAACACAAATAATT
GGGAAAAGATTCAAAAAAGTGCTCTCTTAGGACAAGAGTTGGAGCTGGCAAAGCAAAAGA
TGAAGCTGTTGCAAATCTCAAAGTTGAAGTAACAACAAGACATAGAATTGGTTATTTGGG
GAGGCCAGTTTGAGGATGGAGAAGAGCTTGAGTTTGGTCTTGAAGCACCACAGGACCTCT
TCCAGGTCTGCTGCATATAAAATAACTCACTTCACCTTTTTGATCCTGACTTCCAATTTT
GTCTTTAATTCCTTGTGGAAGCTCTCCTTCCAAGCCTGCCTCTTTTACTATCGATGTATC
CCCCAGTACGTAACTTTACAAATTGACAACTTAGACAACTTAATAACACATTGCGAACGT
TTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCC
TTCACCGCCGGGCCCTAAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGG
CAAAAATCCG
   
Sequence Name 09-K112049-SALK_083793C-R210
GenBank Accession LN484519 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K112049-SALK_083793C-R210
GTGTCATTTGTAAGTTCGTACTGGGGGATACATCGATAGTAAAAGAGGCAGGCTTGGAAG
GAGAGCTTCCACAAGGAATTAAAGACAAAATTGGAAGTCAGGATCAAAAAGGTGAAGTGA
GTTATTTTATATGCAGCAGACCTGGAAGAGGTCCTGTGGTGCTTCAAGACCAAACTCAAG
CTCTTCTCCATCCTCAAACTGGCCTCCCCAAATAACCATTTCTATGTCTTGTTGTTACTT
CAACTCTGAGATTTGCAACAGCTTCATCTTTTGCTTTGCCAGCTCCAACTCTTGTCCTAA
GAGAGCACTCTTCTGAATCTTTTCCCAATTATTTGTGTTTACGTACAATTCTTCTGCATC
GGGTGCAAAAAACAAAACAATCAATATTGCAAACGAATAAGATCTTGATGCTAAATCTGA
GTGTATCATTCAAATCCAGAACAAGAAGAAGTTGTTGTACTGTAGTCCATATCGTATAAA
AACACTAACCATACAAGATCTCGAAACCAAATCATGTGTTGCTGCTTTCACTTGTGACTC
CATCGGAATTCCTGTTTCTTATTTCCCTAGTTTAATCTCTATTCACTGAATTTGATTATT
GTCTATGCACCATGTAGTATTTAATTTGATTCCTCCATCAACTGTTTGTTTTTAGTCCAA
GTTTGCAACTCTTCAATATATGGTTATTATGGTCTTGTGTGACACGAATTATGAATAAGT
TAACTTTGTGCAAGGGAGAAAGTGTGGTTTTTTTTTTATTGGTTGTGTTGTAGGCTGCTG
TAGTAATCCTTTGTTATAGTAATCATATAACTTCCCACGGCAATAAAAACAAGTAAACTT
GGTGATGGAGCCAGATCTATGCTGTA

Last Updated on 06.12.2018 16:11