Sequences from confirmation amplicon


Confirmation sequences for line SALK_083842c and hit At5g20790 are shown.
Line ID SALK_083842c
Locus At5g20790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7040070
Primers used for PCR BL01 - R204, show primer details
   
Sequence Name 34-K104740-SALK_083842C-BL01
GenBank Accession LN485196 [GenBank]
Sequencing primer gene specific primer, BL01: 5'-AATATGTTCGGTTTTCATTTTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K104740-SALK_083842C-BL01
AAGTATATTACTTTTAAAACGATCTATTGTTTCAAGAAATATCAGAAAACAATATTTCTC
AGAGTTGGCTCATTTAAGCTACACGAATAAAGCTGTCGACCAAAAAATATCCGAAAAAAC
TATGTAGCTATGTTAGAGCCAAACTGAGCTAAAAAAAATCCGGGTACGTCGATCAGACCA
GAGTCTAGACTAAGTAAGAAGAATTCGTGGATTTTGCGGGTTTGAGGCTGGGAGTAACAC
GAACAAATACATGCCTACAGGCTACAGTCAAGTTTAAAATGGGCCTAAAAGTAAATGTGT
GTAGTCAATTTTAGATGGGCCTTAACAAGAAAACGCACTTGTTTGTTGTGTTCAAATAGT
TGACCCCTTGTCACGAATTCATCATCGCAATGGACGTGTTACCTAAGTTCGACTAGAAAA
AGCTGGGAATCAACACCAATTGATATTGATTTAAGCGTTCCTTCTTGGTCTCTTGTGTGA
AAATTATTTACAAATTACAACTTAATCAACCAACAAAAAAAAAACACTTCAAACCAAGAA
AGCTTCTCAAGATTTAAAAACTTTCCAAAATATGAAACTTCTCTAATTTAAACACATGCC
GCCGTTACTAGTGTTTTGTTGTCATCCCATAACCTTAATAACACATTGCGGACGTTTTTA
ATGTACTGGGGGGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCAC
CGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTC
   
Sequence Name 91-K104792-SALK_083842C-R210
GenBank Accession LN486338 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K104792-SALK_083842C-R210
CAAAACTAGTAACGGCGGCATGTGTCTAATTAGAGAAGTTTCATATTTCGGAAAGTTTTT
AAATCTTGAGAAGCTTTCTTGGTTTGAAGTGTTTTTTTTTTGTTGGTTGATTAAGTTGTA
ATTTGTAAATAATTTTCACACAAGAAACCAAAAAGGAACGCTTAAATCAATATCAATTGG
GGTTGATTCCCAGCTTTTTCTAGTCAAACTTAGGTAACACGTCCATTGCAATGATAAATT
CGTGACAAGGGGTCAACTATTTGAACACAACAAACAAGTGCGTTTTCTTGTTAAGGCCCA
TCTAAAATTGACTACACACATTTACTTTTAGGCCCATTTTAAACTTGACTGTACCCTGTA
GGCATGTATTTGTTCGTGTTACTCCCAGCCTCAAACCCGCAAAATCCACAAATTCTTCTT
ACTTAGTCTAAACTCTGGTCTGATCGACGTACCCGGATTTTTTTTAGCTCAGTTTGGCTC
TAACATAGCTACATAGTTTTTTCGGATATTTTTTGGTCAACAGCTTTATTCGTGTAGCTT
AAATGACCCAACTCTGAAAAATATTATTTTCTGATATTTCTTTGAAACAATAAATCATTT
TTAAAAGTAATATACTTTTTTACAAATGCAAGTTAAGGCAATGCAAAAATGAAAACCGAA
CATA

Last Updated on 06.12.2018 16:11