Sequences from confirmation amplicon


Confirmation sequences for line SALK_084244c and hit At1g16180 are shown.
Line ID SALK_084244c
Locus At1g16180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5541235
Primers used for PCR 88by - LBb3, show primer details
   
Sequence Name 21-K111273-SALK_084244C-88by
GenBank Accession LN484880 [GenBank]
Sequencing primer gene specific primer, 88by: 5'-CATGCATGATAAACAGAAGGAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K111273-SALK_084244C-88by
GCCAACTGAAACTTGGTATTGATGGTTTACTAGTTCCTTTGTAAGTTGGTCAAAAGTCCG
AAATCAGGAGCGAGATAATAAAGAAACAACGAGTTGTTTTACTTTTCAAGGAGAGGATAG
CTCAAGGTTTTTGATCGATCGCAAGGTATTTTGATTCTCTCTTTCTCAAGCGATTTTCGT
TTTCCTTCTTTTGTTCCAGAGGTCAAATTCAATCCCTAAAGCTAGGTTTCTCGGGTGAAG
CTTTAGATCGGGAGCTATGATTTCATCTCGAATTTGGGGATAAATTTCAGCTTATTTTAA
CCAATTTCAATTGATTCCAAATAATTCTGTTTATCTCTGAGCAAAATTGATTAATTTTCG
ATTCGGTCTTTAAAAAGCTGCTTTTCTTACCGTCTCCATCCGAAATTAGTTTTTTTTGTT
TGTTTGATGATTTGTGTTTTGATGATTTGTGTTTGCGTTTTGGTTGATACTAGCAGCTGA
ATATTGAATCCAACCTTTAATCTGAGAGGAGAAAGAAGATGTTTGCAGCTTCTTGTCTCG
CATCGTGTTGTGCTGCTTGTGCCTGCGATGCTTGCCGTACTGTGGTTTCTGGGATCAGCA
GACGTTCTGCTAGAATTGCTTACTGTGGTCTCTTTGCACTTTCCTTAATCGTTTCATGGA
TTCTCCGTGAAGTTGCTGCTCCTCTCATGGAGAAGCTCCCTTGTAAGTTTTTTTGTTATT
ATACTTCTCGTGAGATTATTTTGGTGTATGCAACTAATCATTGACATTGTGTGCAGGGAT
TAACCATTTTCACAAGACACCTGATCGTGAATGGTTTGAGACTGATGCTGTGTTGCGTGT
CAGCTTAGGGAAAACAAATTGACGCTNAGACAACTTAGTAACACATTGCGGACGTTTTTA
ATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCAC
CGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGGTTGCCCCAGCAGGCGAAA
ATCCCGTTTG
   
Sequence Name 05-K111272-SALK_084244C-R210
GenBank Accession LN484396 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K111272-SALK_084244C-R210
CATTTGTTTTCCTAAGCTGACACGCAACACAGCATCAGTCTCAAACCATTCACGATCAGG
TGTCTTGTGAAAATGGTTAATCCCTGCACACAATGTCAATGATTAGTTGCATACACCAAA
ATAATCTCACGAGAAGTATAATAACAAAAAAACTTACAAGGGAGCTTCTCCATGAGAGGA
GCAGCAACTTCACGGAGAATCCATGAAACGATTAAGGAAAGTGCAAAGAGACCACAGTAA
GCAATTCTAGCAGAACGTCTGCTGATCCCAGAAACCACAGTACGGCAAGCATCGCAGGCA
CAAGCAGCACAACACGATGCGAGACAAGAAGCTGCAAACATCTTCTTTCTCCTCTCAGAT
TAAAGGTTGGATTCAATATTCAGCTGCTAGTATCAACCAAAACGCAAACACAAATCATCA
AAACACAAATCATCAAACAAACAAAAAAAACTAATTTCGGATGGAGACGGTAAGAAAAGC
AGCTTTTTAAAGACCGAATCGAAAATTAATCAATTTTGCTCAGAGATAAACAGAATTATT
TGGAATCAATTGAAATTGGTTAAAATAAGCTGAAATTTATCCCCAAATTCGAGATGAAAT
CATAGCTCCCGATCTAAAGCTTCACCCGAGAAACCTAGCTTTAGGGATTGAATTTGACCT
CTGGAACAAAAGAAGGAAAACGAAAATCGCTTGAGAAAGAGAGAATCAAAATACCTTGCG
ATCGATCAAAAACCTTGAGCTATCCTCTCCTTGAAAAGTAAAACAACTCGTTGTTTCTTT
ATTATCTCGCTCCTGATTTCGGACTTTTGACCAACTTACAAAGGAACTAGTAAACCATCA
ATACCAAGTTTTCATGTTTGGCCCACTAATGGCCCAAATTATTTTAATTTCTCCTTCTGT
T

Last Updated on 06.12.2018 16:11