Sequences from confirmation amplicon


Confirmation sequences for line SALK_084914c and hit At1g72810 are shown.
Line ID SALK_084914c
Locus At1g72810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27400327
Primers used for PCR 54cy - LBb3, show primer details
   
Sequence Name 49-K109566-SALK_084914C-54cy
GenBank Accession LN485475 [GenBank]
Sequencing primer gene specific primer, 54cy: 5'-TTTATCCGCAGGTAAGAAGACAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K109566-SALK_084914C-54cy
AGCGAGAGAGCAGCGGAGGTATCTCCAGTAGAAGCGCAACCAACTCCGATGACTGGTTTG
TTCATTTTCCGGAGACGATTCACCTGAGAAACAAAAAAATAACATAAAGTTGTAAACTTT
ATTGAAGATGGTTATACAGTTGTAAACTTTATGAAAGATGATTCACCTGAGAAACGAGAA
CACTCATGCCCAGATCTTTAAAGGAGCCAGTGTGGCTGATTCCACAATGTTTCACCCAAA
GATCGTTCATTTGCAGATACTGTTTACCAAATCTCTCAGCCCAGAAGAGATTTGAGTTTC
CTTCAAAAGCAGAGACGATGTCGTCATCGTCGATCTCGGGGAGTACCCACTCTTTCTTCG
ACCACACGCCTGATCCGTACGGCCAATTTGTTTTACCGACGCGAGAATCGAAAAGGTTAC
GCCAGAATTCTCCGTCGTAGCGTTTCAAGGCGGCGAAATCATGCTGGACGTCGAGGAGAG
CACCGGATTGTGAACGGTAGACGATCTCGTCGAGGGAGTAGGATTCTGTTGAAGAAGGTG
GAGCGTTGAAAGGGACGTAACGTGCGGAGAGGTTTTGGAGTTGATGAGGACGGCGACGAG
CTTCGTCTCGAATGTTCTCATCGGGTGAACGACGGGGTTTCTGTGGAGTCTGTGGAGGAA
CGGCGGGAGAGGCGGAGGTGCAACGGACGGTGAAGGAAGCGGCGGAGTGAGGTTTAAGTG
AGGTTTCAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTC
TTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCA
   
Sequence Name 40-K109565-SALK_084914C-R210
GenBank Accession LN485292 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K109565-SALK_084914C-R210
CCTCACTCCGCCGCTTCCTTCACCGTCCGTTGCACCTCCGCCTCTCCCGCCGTTCCTCCA
CAGACTCCACAGAAACCCCGTCGTTCACCCGATGAGAACATTCGAGACGAAGCTCGTCGC
CGTCCTCATCAACTCCAAAACCTCTCCGCACGTTACGTCCCTTTCAACGCTCCACCTTCT
TCAACAGAATCCTACTCCCTCGACGAGATCGTCTACCGTTCACAATCCGGTGCTCTCCTC
GACGTCCAGCATGATTTCGCCGCCTTGAAACGCTACGACGGAGAATTCTGGCGTAACCTT
TTCGATTCTCGCGTCGGTAAAACAAATTGGCCGTACGGATCAGGCGTGTGGTCGAAGAAA
GAGTGGGTACTCCCCGAGATCGACGATGACGACATCGTCTCTGCTTTTGAAGGAAACTCA
AATCTCTTCTGGGCTGAGAGATTTGGTAAACAGTATCTGCAAATGAACGATCTTTGGGTG
AAACATTGTGGAATCAGCCACACTGGCTCCTTTAAAGATCTGGGCATGAGTGTTCTCGTT
TCTCAGGTGAATCATCTTTCATAAAGTTTACAACTGTATAACCATCTTCAATAAAGTTTA
CAACTTTATGTTATTTTTTTGTTTCTCAGGTGAATCGTCTCCGGAAAATGAACAAACCAG
TCATCGGAGTTGGTTGCGCTTCTACTGGAGATACCTCCGCTGCTCTCTCTGCTTACTGTG
CTTCCGCTGGAATCCCCTCGATTGTCTTCTTAC

Last Updated on 06.12.2018 16:11